Prostaglandins, Leukotrienes and Essential Fatty Acids
Volume 80, Issue 2 , Pages 165-171 , February 2009

Docosahexaenoic acid is a substrate for ACAT1 and inhibits cholesteryl ester formation from oleic acid in MCF-10A cells

  • Caryl J. Antalis

      Affiliations

    • Cellular Biochemistry Laboratory, Methodist Research Institute, 1800 N. Capitol Avenue, Suite E504, Indianapolis, IN 46202, USA
  • ,
  • Tyler Arnold

      Affiliations

    • Indiana University School of Medicine, Indianapolis, IN 46202, USA
  • ,
  • Bonggi Lee

      Affiliations

    • Department of Foods & Nutrition, Purdue University, West Lafayette, IN 47907, USA
  • ,
  • Kimberley K. Buhman

      Affiliations

    • Department of Foods & Nutrition, Purdue University, West Lafayette, IN 47907, USA
  • ,
  • Rafat A. Siddiqui

      Affiliations

    • Cellular Biochemistry Laboratory, Methodist Research Institute, 1800 N. Capitol Avenue, Suite E504, Indianapolis, IN 46202, USA
    • Indiana University School of Medicine, Indianapolis, IN 46202, USA
    • Corresponding Author InformationCorresponding author. Fax: +13179629369.

Received 21 August 2008 ,Revised 20 November 2008 ,Accepted 3 January 2009.

References 

  1. Chang TY, Chang CC, Cheng D. Acyl-coenzyme A: cholesterol acyltransferase. Annu. Rev. Biochem. 1997;66:613–638
  2. Cases S, Novak S, Zheng YW, Myers HM, Lear SR, Sande E, et al. ACAT–2, a second mammalian acyl-CoA: cholesterol acyltransferase. Its cloning, expression, and characterization. J Biol. Chem. 1998;273:26755–26764
  3. Parini P, Davis M, Lada AT, Erickson SK, Wright TL, Gustafsson U, et al. ACAT2 is localized to hepatocytes and is the major cholesterol-esterifying enzyme in human liver. Circulation. 2004;110:2017–2023
  4. Brown MS, Ho YK, Goldstein JL. The cholesteryl ester cycle in macrophage foam cells. Continual hydrolysis and re-esterification of cytoplasmic cholesteryl esters. J Biol. Chem. 1980;255:9344–9352
  5. Joyce CW, Shelness GS, Davis MA, Lee RG, Skinner K, Anderson RA, et al. ACAT1 and 2 membrane topology segregates a serine residue essential for activity to opposite sides of the endoplasmic reticulum membrane. Mol. Biol. Cell. 2000;11:3675–3687
  6. Rustan AC, Nossen JO, Osmundsen H, Drevon CA. Eicosapentaenoic acid inhibits cholesterol esterification in cultured parenchymal cells and isolated microsomes from rat liver. J. Biol. Chem. 1988;263:8126–8132
  7. Pal S, Davis PJ. Effects of different types of polyunsaturated fatty acids on cholesterol esterification in human fibroblasts. Biochem. Int. 1991;25:281–288
  8. Davis PJ. n-3 and n-6 polyunsaturated fatty acids have different effects on acyl-CoA: cholesterol acyltransferase in J774 macrophages. Biochem. Cell Biol. 1992;70:1313–1318
  9. Seo T, Oelkers PM, Giattina MR, Worgall TS, Sturley SL, Deckelbaum RJ. Differential modulation of ACAT1 and 2 transcription and activity by long chain free fatty acids in cultured cells. Biochemistry. 2001;40:4756–4762
  10. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 1957;226:497–509
  11. Agren JJ, Julkunen A, Penttila I. Rapid separation of serum lipids for fatty acid analysis by a single aminopropyl column. J. Lipid Res. 1992;33:1871–1876
  12. Morrison WR, Smith LM. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J. Lipid Res. 1964;5:600–608
  13. Temel RE, Gebre AK, Parks JS, Rudel LL. Compared with acyl-CoA:cholesterol O-acyltransferase (ACAT) 1 and lecithin:cholesterol acyltransferase, ACAT2 displays the greatest capacity to differentiate cholesterol from sitosterol. J. Biol. Chem. 2003;278:47594–47601
  14. Soule HD, Maloney TM, Wolman SR, Peterson WD, Brenz R, McGrath CM, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 1990;50:6075–6086
  15. Tosi MR, Tugnoli V. Cholesteryl esters in malignancy. Clin. Chim. Acta. 2005;359:27–45
  16. Schonberg SA, Lundemo AG, Fladvad T, Holmgren K, Bremseth H, Nilsen A, et al. Closely related colon cancer cell lines display different sensitivity to polyunsaturated fatty acids, accumulate different lipid classes and downregulate sterol regulatory element-binding protein 1. FEBS J. 2006;273:2749–2765
  17. Batetta B, Pani A, Putzolu M, Sanna F, Bonatesta R, Piras S, et al. Correlation between cholesterol esterification, MDR1 gene expression and rate of cell proliferation in CEM and MOLT4 cell lines. Cell Prolif. 1999;32:49–61
  18. Peiretti E, Dessi S, Mulas C, Abete C, Norfo C, Putzolu M, et al. Modulation of cholesterol homeostasis by antiproliferative drugs in human pterygium fibroblasts. Invest. Ophthalmol. Vis. Sci. 2007;48:3450–3458
  19. Li YC, Park MJ, Ye SK, Kim CW, Kim YN. Elevated levels of cholesterol-rich lipid rafts in cancer cells are correlated with apoptosis sensitivity induced by cholesterol-depleting agents. Am. J. Pathol. 2006;168:1107–1118quiz 1404–1105
  20. MacLean CH, Newberry SJ, Mojica WA, Khanna P, Issa AM, Suttorp MJ, et al. Effects of omega-3 fatty acids on cancer risk: a systematic review. JAMA. 2006;295:403–415
  21. Barascu A, Besson P, Le Floch O, Bougnoux P, Jourdan ML. CDK1-cyclin B1 mediates the inhibition of proliferation induced by omega-3 fatty acids in MDA-MB-231 breast cancer cells. Int. J. Biochem. Cell Biol. 2006;38:196–208
  22. Meuwese MC, Franssen R, Stroes ES, Kastelein JJ. And then there were acyl coenzyme A:cholesterol acyl transferase inhibitors. Curr. Opin. Lipidol. 2006;17:426–430
  23. Nissen SE, Tuzcu EM, Brewer HB, Sipahi I, Nicholls SJ, Ganz P, et al. Effect of ACAT inhibition on the progression of coronary atherosclerosis. N. Engl. J. Med. 2006;354:1253–1263
  24. Fazio S, Major AS, Swift LL, Gleaves LA, Accad M, Linton MF, et al. Increased atherosclerosis in LDL receptor-null mice lacking ACAT1 in macrophages. J. Clin. Invest. 2001;107:163–171
  25. Harris WS, Miller M, Tighe AP, Davidson MH, Schaefer EJ. Omega-3 fatty acids and coronary heart disease risk: clinical and mechanistic perspectives. Atherosclerosis. 2008;197:12–24
  26. Hutter-Paier B, Huttunen HJ, Puglielli L, Eckman CB, Kim DY, Hofmeister A, et al. The ACAT inhibitor CP-113, 818 markedly reduces amyloid pathology in a mouse model of Alzheimer's disease. Neuron. 2004;44:227–238
  27. Huttunen HJ, Greco C, Kovacs DM. Knockdown of ACAT-1 reduces amyloidogenic processing of APP. FEBS Lett. 2007;581:1688–1692
  28. Calon F, Cole G. Neuroprotective action of omega-3 polyunsaturated fatty acids against neurodegenerative diseases: evidence from animal studies. Prostaglandins Leukot. Essent. Fatty Acids. 2007;77:287–293
  29. De Caterina R, Liao JK, Libby P. Fatty acid modulation of endothelial activation. Am. J Clin. Nutr. 2000;71:213S–223S
  30. Kuriki K, Nagaya T, Imaeda N, Tokudome Y, Fujiwara N, Sato J, et al. Discrepancies in dietary intakes and plasma concentrations of fatty acids according to age among Japanese female dietitians. Eur. J. Clin. Nutr. 2002;56:524–531
  31. Philibert A, Vanier C, Abdelouahab N, Chan HM, Mergler D. Fish intake and serum fatty acid profiles from freshwater fish. Am. J. Clin. Nutr. 2006;84:1299–1307

PII: S0952-3278(09)00004-0

doi: 10.1016/j.plefa.2009.01.001

Prostaglandins, Leukotrienes and Essential Fatty Acids
Volume 80, Issue 2 , Pages 165-171 , February 2009