Prostaglandins, Leukotrienes and Essential Fatty Acids
Volume 82, Issue 2 , Pages 97-103, February 2010

Prophylactic and therapeutic effects of Mytilus edulis fatty acids on adjuvant-induced arthritis in male Wistar rats

  • Sarah McPhee

      Affiliations

    • Natural Products Research Group, School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
    • ,
  • Lynn D. Hodges

      Affiliations

    • Natural Products Research Group, School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
    • ,
  • Paul F.A. Wright

      Affiliations

    • Key Centre for Toxicology, School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
    • ,
  • Paul M. Wynne

      Affiliations

    • SGE International Pty Ltd., Ringwood, Victoria 3134, Australia
    • ,
  • Nicolette Kalafatis

      Affiliations

    • Natural Products Research Group, School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
    • ,
  • Theodore A. Macrides

      Affiliations

    • Natural Products Research Group, School of Medical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
    • Corresponding Author InformationCorresponding author. Tel.: +61399257070; fax: +61399257063.

Received 25 February 2009; received in revised form 4 December 2009; accepted 9 December 2009. published online 14 January 2010.

Abstract 

Lipid-rich fractions from the flesh tissue of Mytilus edulis were obtained by solvent extraction and chromatographic separation, and tested for anti-inflammatory (AI) activity in vitro and in vivo. Inhibition of leukotriene production by isolated human neutrophils in response to calcium ionophore stimulation in the presence of exogenous arachidonic acid substrate was demonstrated for the hydrolysed triglyceride fraction of the crude lipid extract. This fraction was subsequently tested for in vivo AI activity using the mycobacterial adjuvant-induced polyarthritis rat model. The hydrolysed triglyceride fraction showed significant AI activity when dosed therapeutically (10mg/kgBW/day, p.o., for 6 days from the onset of arthritis), decreasing body weight loss by 55% and hind paw swelling by 65% compared to the arthritic control. The (non-hydrolysed) crude lipid extract was effective when dosed prophylactically (30mg/kgBW/day, p.o., for 16 days starting on day −2 of arthritigen inoculation). Structural analysis by GC and GC–MS revealed in the extracts an abundance of EPA (20:5n-3) and DHA (22:6n-3) (37% of total fatty acids), along with a small quantity of a rare anti-inflammatory n-3 analogue of arachidonic acid, namely 7, 11, 14, 17-eicosatetraenoic acid (20:4n-3).

Keywords: Anti-inflammatory, Eicosanoids, n-3 PUFA, Mytilus edulis, 7, 11, 14, 17-Eicosatetraenoic acid

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PII: S0952-3278(09)00206-3

doi:10.1016/j.plefa.2009.12.003

Prostaglandins, Leukotrienes and Essential Fatty Acids
Volume 82, Issue 2 , Pages 97-103, February 2010

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