Relationships between seafood consumption during pregnancy and childhood and neurocognitive development_ Two systematic reviews

0952-3278/ Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). T that seafood consumed by pregnant women is likely to benefit the neurocognitive development of their children as described in the accompanying article in this journal [8]. The evidence today is greater with at least 29 published studies evaluating seafood consumption during pregnancy (prenatal exposure) for 106,237 mother-child pairs, and 15 studies of 25,960 children who ate seafood (postnatal exposure). Thus, it is timely and appropriate that the 2020 Dietary Guidelines Advisory Committee is conducting systematic reviews to examine the following questions identified by US Departments of Agriculture (USDA) and Health and Human Services (HHS): Question #40 “What is the relationship between seafood consumption during pregnancy and lactation and the neurocognitive development of the infant?” and Question #41 “What is the relationship between seafood consumption during childhood and adolescence (up to 18 years of age) and neurocognitive development?” https://www.dietaryguidelines.gov/workunder-way/review-science/topics-and-questions-under-review. The Dietary Guidelines for Americans (DGA) systematic review process detailed by the USDA's Nutrition Evidence Systematic Review (NESR) team (https://nesr.usda.gov) is designed to be rigorous and transparent, such that it can be replicated by qualified professionals (https://nesr. usda.gov/2020-dietary-guidelines-advisory-committee-systematicreviews). Our goals were to conduct systematic reviews for these two questions by adhering to the NESR methodology and to contribute the perspectives of an independent technical expert committee with extensive experience on these topics. Critically, the 2020 Dietary Guidelines Advisory Committee uses the term “seafood” in these questions to define the independent or causal variable in these questions as opposed to any single nutritional or chemical component within seafood, whether naturally-occurring or an environmental contaminant. This approach allows development of guidance for the public regarding consumption of seafood as a whole food (e.g., whether to eat seafood during pregnancy and feed seafood to children to benefit their neurocognition) rather than focusing on harms or benefits of individual seafood components. Thus, we systematically reviewed studies that assessed relationships between seafood consumption as a net or whole package because data from these studies more directly and reliably address relationships to neurocognition than studies of individual constituents of seafoods. We also used the 2010 and 2015–2020 DGA definition of seafood as follows: “Seafood is a large category of marine animals that live in the sea and in freshwater lakes and rivers. Seafood includes fish, such as salmon, tuna, trout, and tilapia, and shellfish, such as shrimp, crab and oysters.” [3, 4]. Marine mammals (e.g. porpoises and whales, including pilot whales, which are not commonly consumed by Americans) and sea plants (seaweeds and algae) are not considered to be seafood in this definition. We also considered the importance of the term “relationship” in these questions. We evaluated the overall strength of evidence for whether seafood consumed in pregnancy or childhood is likely to benefit neurocognition, and if so, for the magnitude of those benefits, and whether they are clinically meaningful, lasting, and consistent with the stages of development during which they were examined. Important secondary questions included (a) determination of the lowest and highest amounts of consumption providing benefit, (b) whether there is an optimum beneficial amount, (c) whether some types of seafood are more beneficial than others (e.g. oily or fatty vs. lean or white fish) and (d) whether differentiation by species (e.g. fresh vs. salt water) is merited. Mercury, a neurotoxicant to which the fetus is susceptible, is present at some level in essentially all seafood [9, 10,] so an important question is whether and under what circumstances exposure to mercury from seafood affects neurocognitive outcomes. Nutritional status and mercury exposure could simultaneously influence developmental outcomes in opposite directions. Thus, the examination of seafood as the independent variable simultaneously evaluates the magnitude of adverse effects from exposure to mercury and beneficial effects from nutrients on cognitive development. Considering this, we also sought to determine if any reported levels of seafood consumption resulted in net harms to neurocognition in pregnancy and in childhood. As to be expected, all of the studies that attempted to measure exposure to mercury in addition to maternal seafood consumption reported measures in maternal blood or hair or in cord blood. In describing these findings, hereafter we use the term “mercury” rather than “methylmercury” for consistency because most studies tested for total mercury, which includes methylmercury. 2. Methods Systematic reviews of the evidence relating to the two questions developed by the USDA and HHS for the 2020 Dietary Guidelines Advisory Committee were conducted by a Technical Expert Collaborative (TEC) group, an interdisciplinary team including content matter experts holding advanced degrees in nutrition, medicine, chemistry, or a related field and with experience serving on US and international science-based policy committees (see supplementary materials). All work described in this document was done by members of the TEC; no unnamed staff were involved. The TEC followed the methodology (https://nesr.usda.gov) of the USDA's NESR team (formerly known as the Nutrition Evidence Library), and as described in detail by Obbagy et al. [11]. All TEC members were trained in systematic review methodology as detailed on the 2020 Dietary Guidelines Advisory Committee /NESR website https://nesr.usda.gov/2020dietary-guidelines-advisory-committee-systematic-reviews. Search methodologies including databases and search terms conformed to NESR methodologies. A representative of the TEC clarified questions about the implementation of the systematic methodology and use of the Risk of Bias rating instruments with a representative of the NESR team. The TEC identified both questions to be addressed by the systematic review directly from the 2020 Dietary Guidelines Advisory Committee website https://www.dietaryguidelines.gov/work-under-way/reviewscience/topics-and-questions-under-review. An analytic framework was developed which was applicable to both questions. This analytic framework defined the target population, described seafood exposures and interventions, outcomes, primary confounders and specified key definitions. (Fig. 1) 3. Study criteria The TEC developed a priori criteria for inclusion and exclusion for each of the two systematic methodology questions. To be included, studies needed to be published in English and conducted in very high or high Human Development Index countries [11]. In addition, included studies were required to have one of the following study designs: randomized controlled trial (RCT), prospective cohort study, or case control studies, in which cases were defined as having “neurocognitive disorders and were compared to matched healthy controls”, as described by the NESR team at https://nesr.usda.gov/2020-dietaryguidelines-advisory-committee-systematic-reviews (accessed June 18, 2019). Eligible participants were (for question #40) pregnant women and their offspring, and (for question #41) children who ate seafood between birth to 18 years of all genders. Question #40 was stated so as to evaluate the relationship between maternal seafood consumption and neurocognition in the infant we interpreted “infant” as “offspring” and thus we assessed neurocognitive impacts both in infancy and throughout child development. Included studies were required to have assessed either women who were primarily healthy (i.e., some subjects, but not all, may have had a chronic or pregnancy-related condition) at baseline and/or children who were primarily healthy. Neurocognition was defined as a large category of neurodevelopmental and neuropsychiatric outcomes including IQ measures, cognitive or neuropsychological measures including attention, memory, developmental milestones, hyperactivity, autism, autism spectrum disorder, academic performance, behavior, psychiatric diagnostic J.R. Hibbeln, et al. Prostaglandins, Leukotrienes and Essential Fatty Acids 151 (2019) 14–36


Introduction
Maternal prenatal nutrition and nutrition during childhood are crucial factors in a child's neurodevelopment, and failure to provide adequate amounts of key nutrients at critical periods may result in lifelong impairment in cognitive development and mental health that cannot be corrected by subsequent repletion of nutrients. Seafood is a rich source of key nutrients that are biologically essential for optimal fetal and child neurodevelopment including iodine, vitamin B 12 , iron, vitamin D, zinc, manganese and highly unsaturated omega-3 and omega-6 fatty acids [1]. Women are more likely to the achieve optimal intakes of these nutrients when consuming seafood in pregnancy [2]. T that seafood consumed by pregnant women is likely to benefit the neurocognitive development of their children as described in the accompanying article in this journal [8]. The evidence today is greater with at least 29 published studies evaluating seafood consumption during pregnancy (prenatal exposure) for 106,237 mother-child pairs, and 15 studies of 25,960 children who ate seafood (postnatal exposure).
Thus, it is timely and appropriate that the 2020 Dietary Guidelines Advisory Committee is conducting systematic reviews to examine the following questions identified by US Departments of Agriculture (USDA) and Health and Human Services (HHS): Question #40 "What is the relationship between seafood consumption during pregnancy and lactation and the neurocognitive development of the infant?" and Question #41 "What is the relationship between seafood consumption during childhood and adolescence (up to 18 years of age) and neurocognitive development?" https://www.dietaryguidelines.gov/workunder-way/review-science/topics-and-questions-under-review.
The Dietary Guidelines for Americans (DGA) systematic review process detailed by the USDA's Nutrition Evidence Systematic Review (NESR) team (https://nesr.usda.gov) is designed to be rigorous and transparent, such that it can be replicated by qualified professionals (https://nesr. usda.gov/2020-dietary-guidelines-advisory-committee-systematicreviews). Our goals were to conduct systematic reviews for these two questions by adhering to the NESR methodology and to contribute the perspectives of an independent technical expert committee with extensive experience on these topics.
Critically, the 2020 Dietary Guidelines Advisory Committee uses the term "seafood" in these questions to define the independent or causal variable in these questions as opposed to any single nutritional or chemical component within seafood, whether naturally-occurring or an environmental contaminant. This approach allows development of guidance for the public regarding consumption of seafood as a whole food (e.g., whether to eat seafood during pregnancy and feed seafood to children to benefit their neurocognition) rather than focusing on harms or benefits of individual seafood components. Thus, we systematically reviewed studies that assessed relationships between seafood consumption as a net or whole package because data from these studies more directly and reliably address relationships to neurocognition than studies of individual constituents of seafoods. We also used the 2010 and 2015-2020 DGA definition of seafood as follows: "Seafood is a large category of marine animals that live in the sea and in freshwater lakes and rivers. Seafood includes fish, such as salmon, tuna, trout, and tilapia, and shellfish, such as shrimp, crab and oysters." [3,4]. Marine mammals (e.g. porpoises and whales, including pilot whales, which are not commonly consumed by Americans) and sea plants (seaweeds and algae) are not considered to be seafood in this definition.
We also considered the importance of the term "relationship" in these questions. We evaluated the overall strength of evidence for whether seafood consumed in pregnancy or childhood is likely to benefit neurocognition, and if so, for the magnitude of those benefits, and whether they are clinically meaningful, lasting, and consistent with the stages of development during which they were examined. Important secondary questions included (a) determination of the lowest and highest amounts of consumption providing benefit, (b) whether there is an optimum beneficial amount, (c) whether some types of seafood are more beneficial than others (e.g. oily or fatty vs. lean or white fish) and (d) whether differentiation by species (e.g. fresh vs. salt water) is merited.
Mercury, a neurotoxicant to which the fetus is susceptible, is present at some level in essentially all seafood [9, 10,] so an important question is whether and under what circumstances exposure to mercury from seafood affects neurocognitive outcomes. Nutritional status and mercury exposure could simultaneously influence developmental outcomes in opposite directions. Thus, the examination of seafood as the independent variable simultaneously evaluates the magnitude of adverse effects from exposure to mercury and beneficial effects from nutrients on cognitive development. Considering this, we also sought to determine if any reported levels of seafood consumption resulted in net harms to neurocognition in pregnancy and in childhood. As to be expected, all of the studies that attempted to measure exposure to mercury in addition to maternal seafood consumption reported measures in maternal blood or hair or in cord blood. In describing these findings, hereafter we use the term "mercury" rather than "methylmercury" for consistency because most studies tested for total mercury, which includes methylmercury.

Methods
Systematic reviews of the evidence relating to the two questions developed by the USDA and HHS for the 2020 Dietary Guidelines Advisory Committee were conducted by a Technical Expert Collaborative (TEC) group, an interdisciplinary team including content matter experts holding advanced degrees in nutrition, medicine, chemistry, or a related field and with experience serving on US and international science-based policy committees (see supplementary materials). All work described in this document was done by members of the TEC; no unnamed staff were involved. The TEC followed the methodology (https://nesr.usda.gov) of the USDA's NESR team (formerly known as the Nutrition Evidence Library), and as described in detail by Obbagy et al. [11]. All TEC members were trained in systematic review methodology as detailed on the 2020 Dietary Guidelines Advisory Committee /NESR website https://nesr.usda.gov/2020dietary-guidelines-advisory-committee-systematic-reviews.
Search methodologies including databases and search terms conformed to NESR methodologies. A representative of the TEC clarified questions about the implementation of the systematic methodology and use of the Risk of Bias rating instruments with a representative of the NESR team. The TEC identified both questions to be addressed by the systematic review directly from the 2020 Dietary Guidelines Advisory Committee website https://www.dietaryguidelines.gov/work-under-way/reviewscience/topics-and-questions-under-review. An analytic framework was developed which was applicable to both questions. This analytic framework defined the target population, described seafood exposures and interventions, outcomes, primary confounders and specified key definitions. (Fig. 1)

Study criteria
The TEC developed a priori criteria for inclusion and exclusion for each of the two systematic methodology questions. To be included, studies needed to be published in English and conducted in very high or high Human Development Index countries [11]. In addition, included studies were required to have one of the following study designs: randomized controlled trial (RCT), prospective cohort study, or case control studies, in which cases were defined as having "neurocognitive disorders and were compared to matched healthy controls", as described by the NESR team at https://nesr.usda.gov/2020-dietaryguidelines-advisory-committee-systematic-reviews (accessed June 18, 2019). Eligible participants were (for question #40) pregnant women and their offspring, and (for question #41) children who ate seafood between birth to 18 years of all genders. Question #40 was stated so as to evaluate the relationship between maternal seafood consumption and neurocognition in the infant we interpreted "infant" as "offspring" and thus we assessed neurocognitive impacts both in infancy and throughout child development. Included studies were required to have assessed either women who were primarily healthy (i.e., some subjects, but not all, may have had a chronic or pregnancy-related condition) at baseline and/or children who were primarily healthy.
Neurocognition was defined as a large category of neurodevelopmental and neuropsychiatric outcomes including IQ measures, cognitive or neuropsychological measures including attention, memory, developmental milestones, hyperactivity, autism, autism spectrum disorder, academic performance, behavior, psychiatric diagnostic category (Diagnostic and Statistical Manual (DSM), etc. This definition of neurocognition was consistent with that described during the 2020 Dietary Guidelines Advisory Committee public meeting of March 28-29, 2019. Studies were required to have reported on the relationship between at least one independent variable (seafood consumption) and with at least one dependent variable (neurocognition).
Mercury itself (or Hg chemical forms, e.g. methyl-mercury) has no known beneficial effects on neurodevelopment. Despite this, some studies reported a positive relationship between mercury levels and neurocognitive outcomes. Greater seafood consumption is often positively associated with higher mercury exposure. Therefore, when higher mercury levels were positively associated with cognitive benefits, these mercury levels were highly likely to be reflecting the nutritional effects of seafood thus these studies were also included. Comparators included the consumption of either no seafood or higher vs. lower intakes of seafood. Whenever possible, available data were evaluated to assess "oily" seafood species (e.g. tuna, mackerel, swordfish, salmon, sardines etc.) as compared to "white fish" (e.g. tilapia, cod, pollock, haddock, etc.) We included neurocognitive outcome measures that were age-appropriate, valid and widely accepted for both systematic review questions.

Literature search, screening, and selection
TEC members conducted searches of peer reviewed published literature with date ranges of January 1980-April 2019 in three databases (Cochrane, EMBASE, and PubMed). Search terms defining seafood included seafood, fish, and dietary patterns enriched in seafood. Search patterns for neurocognitive outcomes included developmental milestones, IQ, attention, behavior, social and emotional development, and diagnostic category (e.g. attention deficit hyperactivity disorder) customized for each database. Fig. 2 presents the study selection process. The search plan including the full list of databases and search strategies is available (supplementary materials). To ensure that all relevant articles were identified, a manual search was conducted to find articles that may not have been discovered by our electronic database search. Recommendations were solicited from content matter experts to identify additional articles of potential relevance. Two TEC members independently screened each article's title and/or abstract for relevance using the a priori inclusion and exclusion criteria. Relevant articles were independently screened by two other TEC members at the full-text level. Any disagreements regarding inclusion or exclusion were discussed and resolved among TEC members. The excluded articles and reasons for exclusion are available (supplementary materials). No studies published earlier than 2000 were included, per the DGAC methodology.

Data extraction and risk of bias assessment
The following domains were extracted for articles: study characteristics, participant characteristics, information on the exposure/ independent variables and outcome/dependent variables, confounding variables, statistical adjustments, mercury exposure (if available), results and limitations. At least one additional TEC member verified the completeness and accuracy of the extracted data for quality control. Reported outcomes had to be statistically significant. Studies that reported "weak", "trend" or similarly characterized outcomes, whether trending beneficial or adverse that were not appropriately statistically significant, were defined in this review as being null. TEC members independently assessed the risk of selection, performance, detection,
(literature will be examined by age group, sex, race/ethnicity, and geographic location as appropriate. Age/life stage groups of interest including pregnant or lactating women, children and adolescents).

Intervention/Exposure
Seafood consumption assessed by dietary survey or as an intervention in a controlled study. Mercury as a biomarker of seafood consumption (positive association with neurocognitive outcomes).

Comparator
Consumption of no seafood or different or dissimilar levels of seafood or to a comparison food or mercury as a biomarker of seafood, each assessed as continuous and/or categorical variables.
and attrition biases using the Revised Cochrane risk-of-bias Tool for Randomized Trials https://www.riskofbias.info/welcome/rob-2-0tool/current-version-of-rob-2 or the Risk of Bias-Nutritional Observational Scale (ROB-NOS) adapted by the NESR team for use in nutritional observational studies from the ROBINS-1 https://nesr.usda. gov/2020-dietary-guidelines-advisory-committee-systematic-reviews. Differences in judgements about risk of bias for each article were reconciled by discussion among raters and verification by an independent third rater. Seafood amounts were standardized to ounces/week (oz/ wk) with one meal assumed to be 4 oz unless otherwise defined by the study. Mercury exposures were standardized from blood concentrations to hair mercury using the conversion table in the FDA quantitative assessment of net effects (Table V3 p. 92) [9], with mercury in maternal blood (ug/L) = 3.59* hair mercury (ppm). Cord blood mercury concentrations were standardized to maternal hair mercury using data from Grandjean et al. [12]; cord blood mercury (nmol/L) = 5.0* mercury maternal hair (nmol/g).

Evidence synthesis, conclusion statements, evidence grading and research recommendations
For each systematic review question, the evidence was synthesized qualitatively and graded, conclusion statements were developed, and research recommendations were developed as per NESR methodologies [11]. Briefly, TEC members independently reviewed the extracted data, full-text articles and a description of the body of evidence. Based on the inputs from TEC members, primary TEC members drafted the evidence synthesis including overarching themes and the similarities and differences in findings. A conclusion statement was written to answer each systematic review question, reflecting the synthesis and grading of the available evidence. The TEC used NESR's grading rubric to assign a grade of strong, moderate, limited, or grade not assignable to the evidence underlying each conclusion statement [11]. The grading rubric evaluates internal validity, adequacy, and consistency of the evidence, as well as impact (including clinical impact) and generalizability [11]. TEC members identified research recommendations throughout the process. The conclusion statements developed here were not formulated to make policy recommendations and do not reflect the policy or position of the USDA and HHS, the 2020 Dietary Guidelines Advisory Committee or any Federal or State or private institution. They should not be interpreted to be dietary guidance or advice.

Results
The initial search yielded 2154 articles across neurocognitive outcomes including IQ, verbal development, scholastic achievement, behavior, attention (including risk of attention deficit hyperactivity disorder (ADHD)), autistic phenotypes, cerebral palsy, stereopsis and infant development, including milestones. 994 articles were excluded as duplicates or clinicaltrials.gov citations. 993 articles were excluded based on review of their titles and abstracts and 8 articles were identified by hand search. TEC analysts examined 167 full text articles in detail for inclusion/exclusion and excluded an additional 115 articles (Fig. 2). Common reasons for exclusion were ineligible study design (e.g. cross-sectional studies) and failure to utilize seafood consumption as an independent variable or a parameter of neurodevelopment as a dependent variable (see supplementary materials). For question #40 this process yielded 29 articles comprising 106,237 mother-child pairs, (29 prospective cohort studies) ( Table 1). For question #41 this process yielded 15 articles, comprising 25,960 children (6 RCTs, 4 prospective cohorts, and 9 case control) ( Table 2). These studies were published between 2001 and 2019.  (continued on next page) Mean 0.45 hair ppm Range, 0.03-5.14 ppm Protective associations between higher mercury and better CPT reaction times were found in girls whose mother had < 1 hair ppm.
Maternal seafood consumption > 8 oz/wk was associated with neurocognitive benefits for ADHDrelated outcomes as compared to < 8 oz/wk. Seafood appeared to be protective despite adverse effects of mercury when evaluated as an isolated variable., (comparing ≥ 1 ppm to < 1 ppm. (continued on next page) (continued on next page) 1) large fatty seafood, (such as tuna, swordfish, albacore) 2) smaller fatty seafood, (such as mackerel, sardines, anchovies, salmon" and "tinned sardines/mackerel") 3) lean seafood, (such as hake, sole, or bream"; and "tinned tuna," which has similar levels of DHA and mercury as lean seafood) 4) shellfish, ("such as shrimp, prawns, lobster, or crab"; "clams, mussels, oysters"; and "squid, octopus, cuttlefish") Beneficial associations remained positive through > 30.2 oz/wk of maternal seafood consumption for child neurocognitive development, among a population characterized by high seafood consumption. Intake of small fatty seafood was a predominant predictor of neurocognitive development at 14 mo. and lean and large fatty seafood were predominant predictors of neurocognitive benefits at 5 yrs.
Lower risk of autism-spectrum traits were also observed with total, lean, and large fatty seafood consumption.

Beneficial
Children whose mothers ate oily seafood achieved high-grade stereopsis sooner than those whose mothers did not ( Children whose mothers ate oily seafood were 57% more likely to achieve high-grade stereopsis by age 3.5 yr. (continued on next page) (continued on next page) Although seafood consumption was measured, this study measured the associations between maternal mercury and neurocognition. Higher mercury was associated with better language and receptive communication scores in linear models across the range of mercury levels.
(continued on next page)  (continued on next page) Not able to determine amounts Outcomes categorized by "any fish," "ocean fish," "tuna," and "freshwater fish".
TD= 0.17 SD 0.29 hair ppm AU/SUD =mean 0.14 SD 0.30 hair ppm p=ns Sources of uncertainty included: Differences between groups were not controlled for confounders; and Findings may be due to differing dietary choices among children with AU/ASD rather than due to a cause of AU/ASD (continued on next page) (continued on next page)

Question #40, What is the relationship between maternal seafood consumption during pregnancy and lactation and the neurocognitive development of the infant?
A total of 29 studies were identified for this question . They represent 24 unique cohorts that met the criteria for inclusion and did not meet criteria for exclusion for this question. Of these 29 studies, seven were conducted in the United States [15,20,22,27,32,34,37]; seven in the United Kingdom [13,14,17,19,35,38,39]; three in Spain [23,31,36]; two in Italy [28,29] three in the Republic of the Seychelles [18,25,26]; and one each in Denmark [21], Norway [40], the Netherlands [33], China [30], Japan [24], the Faroe Islands [16] and another in a consortium of European countries [41]. The sample sizes ranged from 135 [15] to 38,581 mother-child pairs [40] with a median sample size of 498 mother child pairs [24].
Overall, the study subjects were healthy and had access to health care. Most of the studies included women between 20 and 40 years of age, with an average age of 29.3 yrs., although many studies included adolescent pregnancies. Fifteen studies described race/ethnicity with an average of 75.5% of the individuals in those cohorts reported being white (range 0-100%). In China [30] and Japan [24] 100% were Asian. Twenty-three studies described maternal education with an average of 58.7% having finished high school (range 15-100%). Thirteen studies reported prevalence of low socioeconomic status with an average of 24% having finished high school (range 8-64%). Twenty studies described any smoking during pregnancy with an average prevalence of 18% (range 4.5-35%). Thirteen studies described drinking alcohol during pregnancy with an average prevalence of 44.6% (range 0-76%). Twelve studies reported "any breastfeeding" with an average of 74.4% (range 10-100%).
Six studies used the FFQ information to report neurocognitive outcomes for oily, or white, or shellfish separately [13,14,19,23,31,39]. Since oily fish are higher in omega-3 fatty acids, differences in outcomes, or lack of differences, between oily and white seafood, could be germane to the contribution that these fatty acids may make to neurocognitive effects. Two studies provided neurocognitive outcomes for canned seafood separately without stating the specific type or species of seafood [28,37]. One study reported neurocognitive outcomes for canned tuna [22]. This was the only study involving maternal consumption that reported outcomes for a specific seafood. Twenty four studies provided neurocognitive outcomes for seafood intake without differentiation among species [13-24, 27-36, 38, 40]. One study used other categorizations of seafood [31].
Of these 29 studies, 24 reported that seafood consumption among mothers was associated with beneficial outcomes to neurocognition on some or all of the tests administered to their children [13-17, 19-27, 29-31, 34-37, 39-41]. The beneficial outcomes appeared on tests administered as early as three days of age and as late as 17 years in age, although nearly all of the testing occurred through age nine (see Table 1).
The five remaining studies reported no significant associations and thus were null for all tests administered [18,28,32,33,38]. Of the five studies reporting completely null results, one was rated as having serious risk of bias on the ROB-NOS due to uncertainty that critical confounding variables were assessed [33] and another one was similarly rated due to reporting unadjusted results as being significant [28] when adjusted analyses were not all statistically significant. Dietary assessment in that study [28] was also problematic due to difficulties in distinguishing prenatal and child intakes None of the studies reported adverse associations between seafood consumption and neurocognitive development.
Higher offspring IQ scores were associated with greater maternal seafood consumption in five studies that measured IQ on a Wechsler Scale of Intelligence (WISC). Gale et al. [19] reported 8.07 points higher verbal IQ scores (95% CI 0.28 to 15.9) among children when comparing maternal consumption of ≥12 oz/wk vs. none. Golding et al. [35] reported that among mothers who ate fish, their children's total IQ averaged 109.3 (SD 1.095) and 99.8 (SD 1.095) in the highest and lowest deciles of mercury exposure respectively. This 9.5 point difference in IQ indicated that greater mercury exposure was not net adverse, provided the mothers ate seafood, and likely indicated that greater seafood consumption increased child IQ. Furlong et al. [37] reported 7.71 higher points on the perceptual reasoning component of IQ, comparing > 8 oz/wk to none. Comparing any seafood vs. no maternal seafood, Lederman et al. [20] reported a 5.6-point increase in verbal and total IQ. Hibbeln et al. [17] reported lower risk of suboptimal verbal and total IQ (OR = 1•48, 95% CI 1•16-1•90) with > 12 oz/wk vs. none. Gains in verbal IQ appear to have provided most of the contribution to total IQ in at least three of these studies [17,19,20]. Consistent with the findings in improved verbal development, Vejrup et al. [40] reported more favorable scores on the Speech and Language Assessment Scale (SLAS), the Ages and Stages Questionnaire (ASQ), and on Twenty Statements about Language-Related Difficulties (language 20) scores when comparing > 14 oz/wk vs. none. These findings were consistent across all levels of fish intake.
Other studies reported improvements in other neurodevelopmental domains but did not find improvements in verbal development. Oken et al. reported beneficial associations to offspring on the Wide Range Assessment of Visual Motor Abilities (WRAVMA) at three years [22] and null associations on the Peabody Picture Vocabulary test at age three and the Kauffman Brief Intelligence Test (KBIT) and WRAVMA scores at age 7.7. Budtz-Jørgensen et al. [16] reported maternal seafood benefits to offspring visual and motor skills, but not to verbal development at 7 and 14 yrs. Steenweg-de Graaff et al. [33] reported null associations with IQ, but did not evaluate verbal IQ or verbal development parameters. Sagiv et al. [27] reported that lower maternal seafood consumption (≤8 oz/wk) as compared to higher consumption (> 8 oz/wk) was associated with greater risk of offspring ADHD diagnoses (e.g. impulsive reactive phenotype, RR = 2.5 95% CI 1.6, 5.0), this despite reporting adverse effects of mercury, (comparing > 1 ppm to < 1 ppm) when assessed as an independent variable separately from seafood. Gale et al. [19] reported that children of mothers not eating oily seafood had nearly three times greater risks of hyperactivity (OR = 2.94, 95% CI 1.28, 6.7) as compared to children whose mothers had eaten oily seafood. Improvements in early childhood neurodevelopment were consistently reported on the McCarthy Scales of Children's Abilities (MSCA) by Mendez et al. [23] (5.9 to 8.6 points, 8-12 oz/wk vs. < 4 oz/wk), Julvez et al. [31] (2.29 points, > 10 oz/wk) and by LLop et al. [36]. Daniels et al. [14] reported scores on the MacArthur Communicative Development Inventory (MCDI) was four points higher comparing offspring of mothers consuming > 18 oz/ wk vs. none. Additionally, in that study scores were higher on the Denver Developmental Screening Test (DDST) with higher seafood consumption (4.5-13.5 oz/wk vs. none). Improvements were reported on the BSID by Julvez et al. [31] for lean and small fish, by Lederman et al. [20] at 36 and 48 mo. and by Lynch et al. [26], Valent et al. [29], and Barbone et al. [41]. Comparing any seafood vs. no maternal seafood, Lederman et al. [20] reported 8.7 higher points in the Bayley Scales of Infant Development Psychomotor Development Index, (PDI). Oken et al. compared the highest quintile of maternal intake (14 oz/wk) to the lowest quintile (1.3 oz/wk) and found greater likelihood of attaining developmental milestones at both six and 18 months (OR = 1.29, 95% CI 1.20, 1.38). Hu et al. [30] found greater maternal seafood to be correlated with better Gesell Developmental Scores in the adaptive domain. Among neonates, Xu et al. [34] reported that greater maternal seafood consumption was associated with "less need for special handling" and "higher asymmetry" scores at five weeks of age while Suzuki et al. [24] reported beneficial associations with motor scores at three days of age on the Neonatal Behavioral Assessment Scale.

Relationships to types of seafood
Regarding oily vs. white or lean seafood, two studies found no differences in outcomes [14,39], while one study found a beneficial association with oily seafood, but not with white [13]. In that study, children of mothers who ate oily seafood were more likely to achieve high grade stereopsis (stereoscopic vision) by 3.5 years of age (adj OR = 1.57, 95% CI 1.00, 2.45) as compared to children whose mothers did not. Another study reported benefits associated with oily seafood, but it is not clear whether oily was more beneficial than white [19]. One study found beneficial associations with oily seafood and with all seafood [31]. Another reported that all seafood (minus squid and shellfish low in the omega-3 fatty acid docosahexaenoic acid (DHA)) was beneficial as compared to consumption of squid and shellfish [23]. Two studies that examined relationships between eating canned seafood (without specifying the species contents) were null and beneficial respectively [28,37], while another reported benefits associated with eating eight or more ounces of canned tuna per week as compared to eating no canned tuna [22].

Relationships to neurocognitive development of seafood through lowest to highest intakes
The lowest and highest levels of seafood consumption in the 29 studies ranged from none to 121 oz/wk [21]. Benefits to neurocognitive development were found at the lowest levels of seafood consumption (i.e., 1.3 oz/wk [34], two oz/wk [13,30], and four oz/wk [21,37] as compared to no consumption). Maternal seafood consumption in a category characterized as ≥12 oz/wk, as compared to lower amounts, was evaluated in nine studies [16, 17, 19, 21-23, 31, 36, 40]. Seven of these reported neurocognitive benefits and two reported neither benefit or harm [23,32]. Oken at al. [22], Llop et al. [36] and Vejrup et al. [40] reported benefits of consumption ≥12 oz/wk despite this intake being associated with higher mercury exposures. Vejrup et al. [40] reported that over the entire range (0-56 oz/wk) of intake, higher maternal seafood consumption was associated with more favorable language and communication scores. Greater seafood intake was highly correlated with higher mercury exposures and this study also found that higher maternal mercury blood levels were associated with greater benefits in three scales of language development. No study reported any adverse effects from maternal consumption of ≥12 oz/wk.Two studies directly reported that the greatest benefits in their cohorts were in consumption categories characterized as ≥ 12 oz/wk [17,19]. One study reported beneficial associations in its highest consumption category with a mean of 14.5 oz/wk [21] while another study reported benefits above 14.1 oz/wk [40]. One study reported beneficial associations > 18 oz/wk [14]. Two studies reported benefits above 30 oz/wk [31,36].
No adverse effects on neurocognitive outcomes were reported from maternal seafood consumption despite very high reported levels of seafood intake, e.g., up to 121 oz/wk [21], 115 oz/wk [13,14,17,35,38,39], 77 oz/wk [24], 65 oz/wk [40], 120 oz/wk [25], 44 oz/wk [29] 32 oz/wk (mean) [36] and 30 oz/wk (mean) [22,31]. Three studies reported that data consistent with a plateau level or asymptotic flattening of the beneficial dose-response relationship whereby after a certain amount, further increases of maternal seafood intake resulted in smaller neurodevelopmental gains. Julvez et al. [31] reported that the highest scores on most tests were in the fourth quintile of seafood consumption in that cohort (mean of 21.2 oz/wk) or in the third/fourth quintiles followed by an attenuation of a positive association in the fifth (highest) quintile (mean 30.2 oz/wk), but still beneficial. In the Avon Longitudinal Study of Parents and Children cohort in the United Kingdom, Daniels et al. [14] and Hibbeln et al. [17] described asymptotic flattening of the beneficial dose response relationship in the highest levels of intake (> 12 oz/wk) for early developmental measures.

Mercury results
Mercury levels from blood or hair obtained from mothers during pregnancy or from umbilical cord blood were reported in 23 of 29 cohorts [14-18, 20, 22, 24-32, 34-36, 38-41]. With the exception of cord [14] and erythrocyte mercury levels [32], we converted results to the equivalent of parts per million (ppm) of maternal hair mercury as described in Table 1. Hu et al. [30] reported the lowest mean mercury exposure (geometric mean 0.2 hair ppm (range < LOD-0.74), arithmetic-mean 0.23 hair ppm (SD 0.11). Davidson et al. [25] reported the highest mean maternal mercury exposure (hair: mean 6.9 ppm, SD 4.4, range 0. 54-22.74). For context, 0.2 ppm is just above the 50th percentile of exposure for women of childbearing age in the United States while 6.9 ppm corresponds to exposure above the 99.9th percentile [9]. Higher mercury levels were associated with improved neurocognitive outcomes in both of these studies [25,30].
Nearly all of the studies that measured mercury exposure treated it as an independent variable and reported associations with neurocognition separately from the associations they reported between seafood and neurocognition. Several studies reported that higher maternal mercury levels were adversely associated with neurocognition when examined independently of seafood. However, in each case seafood consumption itself was beneficially associated with cognitive development [15,16,20,24,39]. In 12 studies, greater maternal seafood consumption had overall beneficial effects for child neurocognitive development despite also measuring exposure to mercury [15,16,18,20,22,[24][25][26][27][39][40][41]. One study attempted to measure the extent to which maternal exposure to mercury affected the association between seafood consumption and neurocognition [22]. That study reported trend data that ≥ 8 oz/wk with less mercury was more beneficial than > 8 ounces per week with more mercury, however these relationships were not statistically significant. One study developed a model suggesting that the benefits DHA (an essential fatty acid rich in seafood) decreased as exposure to mercury approached nine and 11 ppm in hair depending on the neurocognitive test. There was no benefit beyond these levels. Both levels are substantially above the 99.9th percentile of exposure in U.S. women of childbearing age [26]. We mention this study because it is the only one we identified that provided evidence for a mitigating effect of mercury on the benefits associated with a nutrient found in seafood (DHA).
Six studies reported null associations between maternal mercury levels and neurocognition [28-30, 32, 33, 38]. Seven studies reported seemingly paradoxical findings, that higher levels of mercury had beneficial relationships to neurocognitive development [25, 27, 34-36, 40, 41]. For example, in one study a doubling in mercury was associated with higher scores in most of the McCarthy Scales of Child Abilities scales [36]. As noted earlier, this result is likely due to higher maternal mercury levels acting as an indicator of greater seafood consumption.
The United States Environmental Protection Agency (EPA) Reference Dose (RfD) of 1.1 ppm in maternal hair was exceeded in 22 studies [14-18, 20, 22, 24-29, 31, 32, 34-36, 38-41]. Eight of these studies had mean exposures above 1.1 ppm [16,18,24,25,31,36], and all the studies had participants with exposures that were many times higher than the RfD. None of these studies reported any net adverse effects on neurocognitive development from seafood consumption in any amount.
Evaluation of criteria for the conclusion statement, question #40 The conclusion statement below was developed based on the following evaluation criteria of elements in the USDA NESR conclusion statement (https://nesr.usda.gov).

Element 1, Risk of Bias -Grade II, Moderate
Nearly all studies met the criteria for having "moderate" risk of bias utilizing the instruments and procedures described by the NESR [11]. The most common reason for failure to meet criteria for low risk of bias was the inability to be completely certain that no residual confounding was present, an inherent characteristic of all observational studies. Assessment of evidence as moderate rather than strong reflected the fact that the evidence is based on observational studies, rather than well designed RCTs. An RCT that randomized pregnant women to a no-fish control group over an entire pregnancy would be contrary to the current Dietary Guidelines for Americans recommendation for fish intake.

Element 3, Consistency -Grade 1: Strong
Twenty-five of the 29 studies reported beneficial associations between seafood and neurocognitive development with good consistency throughout neurocognitive development utilizing well validated, age appropriate instruments. None of the 25 studies reporting benefits were judged to have serious risk of bias using the ROB-NOS. In contrast, two of the five studies reporting null effects on all outcomes were judged to have serious risk of bias. No studies reported net adverse effects.

Element 4, Impact -Grade 1: Strong
In all cases the studied outcomes related directly to the systematic review question and in most cases the size of effects was clinically meaningful or probably clinically meaningful. For example, the gains in IQ in these studies ranged from 5.6 to 9.5 IQ points. In comparison, breastfeeding results in benefit for full term infants of 2.66 higher IQ points after adjusting for maternal intelligence [44].

Element 5, Generalizability-Grade 1: Strong
The studies provided data directly from seven U.S. cohorts and 18 European cohorts generalizable to U.S. populations. The characteristics of the study populations with regards to age, education, occupational status, family characteristics, etc. closely aligned with U.S. characteristics. The cohorts in the Republic of the Seychelles consumed large amounts of seafood (i.e., a mean of 48 oz/wk), resulting in high mercury exposures (averages of 5.9 and 6.9 ppm maternal hair). The mercury concentrations of seafood consumed in the Seychelles were similar to that of seafood available in the United States and thus generalizable to U.S. commercial species in that respect, while sea mammals such as pilot whales were not consumed [18]. In addition, the amounts of seafood consumed and the lower exposures to mercury in the Seychelles overlap with high end amounts and exposures in the United States [9]. Consequently, these findings can be generalized to high consumption populations within the U.S. However, overall generalizability of the 29 studies would have been improved with greater inclusion of more low income or socially vulnerable populations and populations that depend on recreational and subsistence catch.
Overall, these studies were conducted with subjects who were all healthy, or healthy subjects without ADHD or developmental disabilities were used as comparators. All participants had access to health care. Rates of obesity among participants was generally very low, typically < 2%. Parent education was high with an average of 34.8% terminating with high school and 46.7% with completing higher education degrees. Two studies conducted in China [51,58] and one in Korea [57] had no Caucasians. Among the remainder of the studies, were 89% white and non-immigrant. Auberg et al. [45] studied Swedish military conscripts, 100% of which were male. The remainder of the studies had an average of 54% males. Hertz-Picciotto et al. [47] studied children with autism or autism spectrum disorder of whom 75% were male, reflecting the normal sex distribution of the disorder. Ages averaged 9.3 years across all studies with a range of 18 mo. [14] to 16 years [49].

Neurocognitive outcomes question #41
Thirteen of the 15 manuscripts reported beneficial neurocognitive outcomes associated with consumption of seafood among children [14, 45-47, 49-54, 56-58]. Two reported findings that were null [48,55] and none reported adverse outcomes. Beneficial relationships with measures of intelligence were reported in the two largest prospective cohort trials (school grades at 16 years of age [49] and standardized intelligence tests [45]) and a smaller prospective cohort (WISC-III [51]) among children 12 years old. In that study, verbal IQ gained 4.75 points while full scale IQ scores gained 4.8 points when seafood consumption was ≥4 oz/wk compared to less seafood. Seafood consumption at 15 mo. was associated with better MCDI scores at 18 mo. [14]. In four studies, greater seafood consumption [53,58] or dietary patterns characterized by seafood consumption [54,57] were associated with lower risks for a diagnosis of Attention Deficit Hyperactivity Disorder using Diagnostic Statistical Manual criteria. One case-control study reported that seafood consumption was associated with lower risk of autism or autism spectrum disorder [47], but the study was judged to have a serious risk of bias due to inadequate control of confounding variables and the possible influence of diagnosis on dietary choices.

Relationships to types of seafood
Two studies suggested that oily seafood as compared to white seafood may be somewhat protective against risk of ADHD-related disorders, although the results might simply represent dietary preferences among children with and without ADHD-related disorders [53,57]. In three RCTs, children eating oily seafood meals had greater improvement on test scores as compared to meat meals [46,50,52] and in one of these, oily seafood meals were more beneficial than omega-3 supplements [46]. These studies did not compare oily seafood to white seafood, however. No other study reported outcomes for specific species. No studies provided neurocognitive outcomes for canned fish separately.

Relationships to neurocognitive development of seafood at lowest to highest intakes
Five studies reported benefits [14,45,47,49,51] when comparing seafood consumption above one meal/wk (4 oz/wk). Two studies [53,54] reported that an intake of > 8-12 oz/wk was associated with the greatest benefits. Insufficient data were reported on ranges, gradients and upper quantities of seafood consumed to make any assessment of dose response relationships or conclusions regarding maximal levels of benefit. Only one study characterized results by any differentiation of oily/fatty seafood and white seafood [53] finding that fatty seafood was more protective than white seafood.

Mercury results
Two studies reported measurements of mercury exposure in the children [47,50]. In Kvestad et al. consumption for seafood in an RCT caused mercury to increase from 0.373 ppm to 0.520 ppm [50]. Despite this elevation in mercury, neurocognitive benefits were reported. Hertz-Picciotto et al. , reported that seafood consumption was associated with higher mercury levels, which were nearly twice as high in children with "typical development" (0.29 ppm) as compared to children with symptoms of autism spectrum disorder (0.14 ppm). Although mercury levels were not reported in the remainder of the studies, it is highly likely that greater seafood consumption in those studies resulted in higher mercury exposures. Since no study reported net adverse outcomes from seafood consumption in children, it is unlikely that mercury exposure from seafood was associated with substantive neurocognitive harms.
Evaluation of criteria for the conclusion statement, question #41 The conclusion statement below was developed based on the following evaluation criteria of elements in the USDA NESR conclusion statement (https://nesr.usda.gov).

Element 1, Risk of Bias -Grade II, Moderate
Nearly all studies met criteria for having "moderate" risk of bias utilizing the instruments and procedures described by the NESR [11]. The most common reason for failure to meet criteria for low risk of bias was inability to be certain of no residual confounding, an inherent characteristic of all observational studies. Seven reports from four RCTs were published, These RCTS were moderately sized (n = 183, 232, 426 and 726). Three [48,50,52] reported results from the FINS-KIDS Study and two [46,55] from the FINS-TEENS Study.

Element 3, Consistency -Grade 1: Strong
Thirteen of the 15 papers reported beneficial neurocognitive outcomes associated with consumption of seafood among children using age appropriate well validated instruments [14,[45][46][47][49][50][51][52][53][54][55][56][57][58] The two studies that reported gains in IQ are in addition to the five that reported IQ gains in association with maternal consumption [45,51]. Two studies reported null findings on some outcomes [48,55] although beneficial results for other aspects of neurocognitive development were reported in studies of the same cohorts. No studies reported adverse outcomes.
Element 4, Impact -Grade 1: Strong In all cases the studied outcomes related directly to the systematic review question and in most cases the size of effect was clinically meaningful (e.g., the gains in IQ and lower risks of ADHD).

Element 5, Generalizability-Grade 1: Moderate
The studies provided data from cohorts generalizable to U.S. populations, e.g., one U.S. and 10 European cohorts. Children were healthy and had good access to medical care. The characteristics of the study populations with regards to age, education, occupational status, family characteristics, etc. aligned with U.S. characteristics. However, non-white populations were under-represented as compared to the U.S. population.

Discussion
Here we followed the systematic review methodology detailed by the USDA's NESR team (https://nesr.usda.gov) to evaluate two questions to be addressed by the 2020 SAC-Dietary Guidelines for Americans and contribute perspectives from a technical expert committee with extensive topical experience. We conclude that there is moderate and consistent evidence that seafood consumption both during pregnancy and childhood benefits neurocognitive development. The magnitude of effects was clinically significant. In the seven studies that reported gains in IQ, the gains ranged from 4.8 points [51] to 9.5 IQ points [35] when seafood was consumed in the highest consumption categories in those cohorts. Such gains could be significant on an individual and population-wide basis. Thirteen studies reported as beneficial the consumption of ≥12 oz/wk or categories that included consumption > 12 oz/wk [14-17, 19, 21, 22, 24, 25, 27, 31, 36, 40]. Two studies reported the consumption of > 12 oz// wk was not associated with harm [23,32]. Llop et al. [36] and Vejrup et al. [40] also reported benefits of consuming ≥12 oz/wk despite also finding associations between greater seafood consumption and higher mercury levels. In 10 of these studies, consuming ≥12 oz/wk was more beneficial that consuming < 12 oz/wk for at least some outcomes [14,16,17,19,21,22,27,31,36,40].
One of the most significant outcomes of this systematic review is the absence of evidence for any net adverse effects of seafood on neurocognitive development even at the highest levels of intake. We found no evidence to support an upper limit of 12 oz/wk of commercial seafood (i.e., evidence that exceeding this intake was associated with harm). Three studies that provide evidence that consuming between 12 and 20 oz/wk amounts provide maximum benefits [14,17,31]. The United States Environmental Protection Agency (EPA) Reference Dose (RfD) of 1.1 ppm in maternal hair was exceeded in 22 studies, often by many times [14-18, 20, 22, 24-29, 31, 32, 34-36, 38-41]. Despite this, we found that consuming seafood during pregnancy and childhood was likely beneficial and clearly not adverse to neurocognition. Higher mercury levels were associated with benefits to neurocognitive outcomes in seven studies with 45,957 mother infant pairs, likely indicating greater seafood consumption [25, 27, 34-36, 40, 41]. These studies corroborate that the US EPA RfD is a level of exposure deemed to be without appreciable risk [10].
The published studies typically did not qualify species of seafood consumed making it impossible to evaluate effects of specific species or types of seafood on neurocognition. We could find no data regarding effects on neurocognitive development from consuming species of seafood distinguished by varying amounts of mercury by any quantitative definition (e.g., "low", "moderate" and "high" mercury seafood). There was insufficient direct data to form any conclusions regarding freshwater non-commercial species; none of the studies we reviewed distinguished freshwater non-commercial catch from commercially available seafood or wild caught from aquacultured species, however, a reasonable assumption is that the majority of the seafood consumed was commercially available and included a substantial amount of aquacultured seafood. Aquacultured seafood tend to be toward the low end in terms of mercury because they are grown rapidly without much opportunity to accumulate mercury [9]. Finally, most studies did not report seafood preparation, however, one study did report an adverse association between fried seafood and neurocognition [31].

Research recommendations
TEC members identified research gaps and offered the following recommendations: 1) Conduct more prospective cohort research in diverse populations within and outside of the United States. 2) Use standardized outcome measures when possible (e.g. IQ or IQ equivalent scores, especially for verbal development), so that outcomes can be consistently scored, compared, and reproduced across studies. 3) More detailed recording of species and preparation. 4) Conduct studies to develop a better understanding of whether seafood containing greater quantities of omega-3 fatty acids, i.e., oily or fatty seafood, is more beneficial than seafood containing less, i.e., white seafood. 5) Assess contributions of genetic variants and their interactions with dietary intakes. 6) Conduct adequately powered RCTs when possible to better support causal inferences. 7) Conduct more studies on effects from maternal seafood consumption beyond nine years of age. 8) Conduct studies on the effects on neurocognition from non-commercial catch from rivers, lakes, and streams.

Conclusion statement question #40
After reviewing the evidence, the TEC members developed the following conclusion statement to answer the question #40 assessing the relationship of seafood consumption during pregnancy and lactation to neurocognitive development of the infant: "Moderate and consistent evidence indicates that consumption of a wide range of amounts and types of commercially available seafood during pregnancy is associated with improved neurocognitive development of offspring as compared to eating no seafood". This evidence does not meet the criteria for "strong" evidence only due to the absence of randomized controlled trials that may not be ethical or feasible to conduct. Overall, benefits to neurocognitive development began to appear at the lowest amounts of seafood consumed (∼4 oz/wk) and continued into the highest categories of consumption in those cohorts (> 100 oz/wk). Benefits consistently increased from no seafood consumption upwards through approximately > 12-30 oz/wk. After those levels of consumption, benefits continued to be present. The TEC could find no upper limit of seafood intake that resulted in adverse outcomes for any measure of neurocognitive development as compared to eating no seafood or less seafood even though the highest amounts exceeded current average intake of Americans (< 5 oz/wk) by more than 20-fold (> 100 oz/wk). Seafood provided overall benefits to neurocognitive development even when mercury exposures in the same study populations were high by U.S. standards. In some studies, oily fish (tuna, salmon, swordfish, sardines, etc.) showed benefits when white fish and shellfish did not, however data are insufficient from these studies overall for a conclusive statement regarding types of seafood or specific species that convey the greatest benefits. Most of the research was conducted in healthy women and offspring consistent with U.S. population demographics. Evidence is insufficient to assess the relationship between seafood consumption during lactation and neurocognitive development."

Conclusion statement question #41
After reviewing the evidence, the TEC members developed the following conclusion statement to answer the question #41 assessing the relationship of seafood consumption during childhood and adolescence (up to 18 years of age) and neurocognitive development: "Moderate and consistent evidence indicates that consumption of > 4 oz/wk and likely > 12 oz/wk of a wide range of commercially available seafood during childhood through adolescence has beneficial associations to a wide spectrum of neurocognitive outcomes as compared to consuming no seafood. The evidence does not meet the criteria for "strong evidence because of an insufficient number of randomized controlled trials. The TEC could find no amount of seafood in these studies that resulted in net adverse outcomes for any measures of neurocognitive development in children; however upper ranges of consumption were not well described. Seafood provided overall benefits to neurocognitive development and exceeded potential harms from mercury in seafood. However, specific levels of mercury exposure were often not reported. Data were insufficient for a conclusive statement regarding neurocognitive effects from types of seafood or specific species. Most of the research was conducted in healthy children reasonably consistent with U.S. population demographics."

Declaration of Competing Interest
The following authors have no conflicts to declare, JRH, PS, JTB, JG, BJH, PK-E, BL, SLC, GM, JJS, MAC, SEC. WS Harris is the President of OmegaQuant Analytics, LLC a laboratory that offers fatty acid testing for researchers, clinicians and consumers. The corresponding author does not consider this to be a conflict of interest.