Research Article| Volume 11, ISSUE 4, P419-430, August 1983

Effects of bradykinin and bovine serum albumin on arachidonic acid and prostaglandin release from perfused rat heart

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      The isolated perfused rat heart was used to study if arachidonic acid (AA) release affected prostaglandin (PG) production. The perfused heart avidly absorbed and esterified exogenous AA, mostly into polar lipids. The administration of bradykinin (BK) after prelabeling with 1-C14-AA caused a two-fold increase in the amount of AA released. Bovine serum albumin (BSA) stimulated release 2.5-fold. Perfusion with AA saturated BSA enhanced the release of 1-14C-AA 23-fold indicating the presence of an active deacylation/reacylation system in rat heart.
      BK caused a four-fold increase whereas BSA had no effect on the amount of prostaglandins released from perfused heart indicating that BK acted on a specific AA pool available to cyclooxygenase whereas the BSA stimulated a separate pool of AA which apparently was not available to the cyclooxygenase.
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        • Nelson N.A.
        • Kelley R.C.
        • Johnson R.A.
        Prostaglandins and the arachidonic acid cascade.
        Chem. Eng. News. 1982; 60: 30-44
        • McGiff J.
        New developments in prostaglandin and tromboxane research.
        in: 5th Ed. Federal Proceedings. 38. 1979: 64-93
        • Lands W.
        The biosynthesis and metabolism of prostaglandins.
        Ann. Rev. Physiol. 1979; 41: 633-652
        • Terrangno N.A.
        • Lonigro A.J.
        • Malik K.
        • McGiff J.
        The relationship of the renal vasodilator action of BK to the release of PGE-like substances.
        Experientia. 1972; 28: 437-439
        • Vogt W.
        • Meyer U.
        • Luft E.
        • Babilli S.
        Formation of phospolipase A2 of SRS-C in perfused guinea pig lung. Identification with PG.
        N-S Archi. Pharm. K. 1969; 262: 124
        • Isakson P.
        • Raz A.
        • Needleman P.
        Selective incorporation of 14C-arachidonic acid into the phospholipids of intact tissues and subsequent metabolism to 14C-PG's.
        Prostaglandin. 1976; 12: 739-748
        • Isakson P.
        • Raz A.
        • Denny S.
        • Wyche A.
        • Needleman P.
        Hormonal stimulation of AA release from isolated perfused organs: Relationship to PG biosynthesis.
        Prostaglandin. 1977; 14: 853-870
        • Hsueh W.
        • Needleman P.
        Sites of lipase activation and PG synthesis in isolated perfused rabbit hearts and kidneys.
        Prostaglandin. 1978; 16: 661-681
        • Hsueh W.
        • Isakson P.
        • Needleman P.
        Hormone selective lipase. Activation in the isolated rabbit heart.
        Prostaglandin. 1977; 13: 1073-1091
        • Spector A.A.
        • John K.
        • Fletcher J.
        Binding of long chain fatty acids to bovine serum albumin.
        Journal Lipid Res. 1969; 1: 56-67
        • Zachariah P.
        Contractility and sugar permeability in perfused rat heart.
        J. Physiol. 1961; 158: 59
        • Folch J.
        • Lees M.
        • Sloane-Stanley G.H.
        A simple method for isolation and purification of total lipids from animal tissues.
        Journal Biol. Chem. 1957; 226: 497-509
        • Kinsella J.E.
        Incorporation of 14C-glycerol into lipids by dispersed bovine mammary cells.
        Biochimica et Biophysica Acta. 1968; 164: 540-549
        • Yu P.H.
        • Kinsella J.E.
        The effects of dietary trans, trans methyl octadecadienoate acid on composition and fatty acids of rat heart.
        Journal Am. Clinical Nutrition. 1980; 33: 598-605
        • Goswami S.K.
        • Kinsella J.E.
        Separation of prostaglandin A,B,D,E,F, thromboxane and 6 Keto prostaglandin F by thin-layer chromatography.
        Journal Chromatog. 1981; 209: 334-336
        • Snedecor G.
        • Cochran G.
        Statistical Methods.
        5th Ed. Iowa State Press, Ames, Iowa1979
        • Needleman P.
        • Marshall G.
        • Sobel B.
        Hormone interactions in the isolated rabbit heart.
        Circ. Res. 1975; 37: 802-808
        • Nasjletti A.
        • Malik K.
        Relationship between the kallikreinkinin and prostaglandin systems.
        Life Sciences. 1979; 25: 99-110
        • DeGrella R.
        • Light R.
        Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. I. Kinetics of homologues fatty acids, and II. Inhibition of albumin and fatty acid homologues and the effect of temperature and metabolic reagents.
        J. Biol. Chem. 1980; 225: 9739-9745
        • Spector A.A.
        The transport and utilization of free fatty acids.
        NY Acad. Sci. 1969; 149: 768-783
        • Hong S.
        • Levine L.
        Stimulation of PG by BK and thrombin and their mechanism of action on MC5-5 fibroblasts.
        J. Biol. Chem. 1976; 251: 5814-5816
        • Evans J.
        • Opie L.
        • Shipp J.
        Metabolism of palmitic acid on perfused rat heart.
        Am. J. Physiol. 1963; 205: 766-770
        • Hiroko H.
        • Ohngk R.
        Extraction and partial purification of acyl-CoA acyl-sn-glycero-3-phosphocholine acyltransferase from rat liver microsomes.
        BBA. 1980; 617: 205-217
        • Chern J.C.
        • Kinsella J.E.
        Concurrent release and reacylation of fatty acids by rat kidney cells: Selective incorporation of arachidonic acid. Biochim.
        Biophys. Acta. 1982; (In Press)
        • Raz A.
        • Schwartzman M.
        Time-synchronized activation of lipolysis and fatty acids; reacylation by bradykinin and angiotensin in the perfused rabbit kidney.
        Adv. PG Thromboxane Res. 1980; 6: 255-258
        • Schwartzman M.
        • Liberman E.
        • Raz A.
        BK and angiotensor II activation of AA deacylation and PG formation in rabbit kidney.
        J. Biol. Chem. 1980; 256: 2329-2333
        • Erman A.
        • Azuri E.
        • Raz A.
        Enzymatic coupling of acylhydrolase and prostaglandin synthetase activities in sub-cellular fractions from rabbit renal medulla.
        Biochem. J. 1982; 201: 635-640
        • Robinson P.
        • Bastian D.
        • Hamer P.
        • Pichiarallo D.
        • Stephanson M.
        Mechanisms of stimulation of PG synthesis by a factor from rheumatoid synovial tissue.
        in: Proc. Nat. Acad. Sci.78. 1981: 5160-5164 (8)
        • Waite M.
        • Daniel L.
        • Beaudry G.
        The source of arachidonate from prostaglandin synthesis by MDCK cells.
        Prev. & Related Lipids. 1982; 2: 265-271