Advertisement
Research Article| Volume 11, ISSUE 4, P431-442, August 1983

The effects of insulin, glucose and diabetes on prostaglandin production by rat kidney glomeruli and cultured glomerular mesangial cells

DOI:https://doi.org/10.1016/0262-1746(83)90097-5
The effects of insulin, glucose and diabetes on prostaglandin production by rat kidney glomeruli and cultured glomerular mesangial cells
Previous ArticleEffects of bradykinin and bovine serum albumin on arachidonic acid and prostaglandin release from perfused rat heart
Next ArticleDietary arachidonic acid supplementation increases thromboxane synthesizing activity in platelets from diabetics
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Glomeruli isolated from streptozotocin-diabetic rats produced significantly greater amounts of immunoreactive prostaglandin (PG)E2, PGF2α, and prostacyclin (PGI2) measured as the stable metabolite 6-keto-PGF1α than control glomeruli. These data led to studies to determine whether the vasoactive glomerular mesangial cell exhibited alterations in arachidonic acid metabolism in diabetes. Therefore, we isolated and cultured under identical conditions, mesangial cells from normal and streptozotocin-diabetic rats. Normal mesangial cells produced predominantly PGE2 (57–72%) with PGE2 > PGF2α > PGI2 after stimulation of acylhydrolase with melittin. Mesangial cells from diabetic rats produced predominantly PGI2 (55–73%) with PGI2 > PGE2 > PGF2α. A similar prostaglandin profile was obtained when arginine vasopressin (AVP) was used to stimulate acylhydrolase activity. In addition, diabetic mesangial cells synthesized greater amounts of prostaglandins than normal mesangial cells cultured for the same number of passages. When cultured under high-glucose conditions (in tissue culture medium with a final glucose concentration of 550 mg/dl) to mimic the diabetic state Math Eq, normal mesangial cell's produced proportionately greater amounts of PGE2, PGF2α and PGI2; no alteration to predominantly PGI2 production was observed. Insulin addition to the high-glucose condition tended to attenuate prostaglandin production. Diabetic mesangial cells likewise produced more prostaglandins when cultured under high-glucose conditions; however, the increases were not proportional among the 3 prostaglandins examined. PGE2 production increased to a greater degree than PGI2. With insulin present in the high-glucose condition, there was a disproportional attenuation of all prostaglandins produced, with PGI2 decreasing more than PGE2. Thus, the streptozotocin-induced diabetic state resulted in an alteration in mesangial cell arachidonic acid metabolism.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Prostaglandins, Leukotrienes and Essential Fatty Acids
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Halushka P.V.
        • Lurie D.
        • Colwell J.A.
        Increased synthesis of prostaglandin E-like material by platelets from patients with diabetes mellitus.
        New England Journal of Medicine. 1977; 24: 1036
        • Gerrard J.M.
        • Stuart M.J.
        • Rao G.H.R.
        • Steffes M.W.
        • Mauer S.M.
        • Brown D.M.
        • White J.G.
        Alteration in the balance of prostaqlandin and thromboxane synthesis in diabetic rats.
        Journal Laboratory Clinical Medicine. 1980; 95: 950
        • Tannebaum J.
        • Niles A.S.
        • Aulsebrook K.
        • Sweetman B.
        • Oates J.A.
        Release of prostaglandins from rat renal papilla Math Eq: Effect of glucose concentration.
        in: Federation Proceedings. 35. 1976: 223 (abstract)
        • Mogensen C.E.
        Glomerular filtration rate and renal plasma flow in shortterm and long-term juvenile diabetes.
        Scandinavian Journal of Clinical Laboratory Medicine. 1971; 28: 91
        • Carney S.L.
        • Wong N.L.M.
        • Dirks J.H.
        Acute effects of streptozotocin diabetes on rat renal function.
        Journal Laboratory Clinical Medicine. 1979; 93: 950
        • Ausiello D.A.
        • Kreisberg J.I.
        • Roy C.
        • Karnovsky M.J.
        Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin.
        Journal Clinical Investigation. 1980; 65: 121
        • Sraer J.
        • Foidart J.
        • Chansel D.
        • Mahieu P.
        • Kouznetzova R.
        • Ardaillou R.
        Prostaglandin synthesis by mesangial and epithelial glomerular cultured cells.
        FEBS Letters. 1979; 104: 420
        • Kreisberg J.I.
        • Karnovsky M.J.
        • Levine L.
        Prostaglandin production by homogeneous cultures of rat glomerular epithelial and mesangial cells.
        Kidney International. 1982; 22: 355
        • Scharschmidt L.
        • Dunn M.
        • Aikawa M.
        • Hassid A.
        Vasopressin (AVP)-entranced PGE2 production by cultured rat glomerular mesangial cells.
        Kidney International. 1982; 21 (abstract): 265
        • Uglesity A.
        • Kreisberg J.I.
        • Levine L.
        Stimulation of arachidonic acid metabolism in rat kidney mesangial cells by bradykinin, antidiuretic hormone, and their analogues.
        Prostaglandins, Leukotrienes, and Medicine. 1983; 10: 83
        • Burlington H.
        • Cronkite E.P.
        Characteristics of cell cultures derived from renal glomeruli.
        in: Proceedings Society Experimental Biological Medicine. 142. 1973: 143
        • Foidart J.B.
        • Dechenne C.A.
        • Mahieu P.
        • Crentz C.A.
        • De Mey
        Tissue culture of normal rat glomeruli. Isolation and characterization of the homogeneous cell lines.
        Investigation and Cell Pathology. 1979; 2: 15
        • Barnes L.D.
        • Guy M.N.
        • Lifschitz M.D.
        • Kreisberg J.I.
        Angiotensin II receptors in mesangial cells cultured from rat renal glomeruli.
        Kidney International. 1980; 19 (abstract): 163
        • Foidart J.
        • Sraer J.
        • Delarue F.
        • Mahieu P.
        • Ardaillou R.
        Evidence for mesangial glomerular receptors for angiotensin II linked to mesangial cell contractility.
        FEBS Letters. 1980; 121: 333
        • Kreisberg J.I.
        • Hoover R.L.
        • Karnovsky M.J.
        Isolation and characterization of rat glomerular epithelial cells Math Eq.
        Kidney International. 1978; 14: 21
        • Levine K.
        • Gutierrez-Cernosek R.M.
        • Van Vunakis H.
        Specificities of prostaglandins B1, F1α, and F2α.
        Journal Biological Chemistry. 1971; 246: 6782
        • Alam I.
        • Ohuchi K.
        • Levine L.
        Determination of cyclooxygenase products and prostaglandin metabolites using high-pressure liquid chromatography and radioimmunoassay.
        Analytical Biochemistry. 1979; 93: 339
        • Levine L.
        • Alam L.
        Arachidonic acid metabolism by cells in culture: Analysis of culture fluids for cyclooxygenase products by radioimmunoassay before and after separation by high-pressure liquid chromatog raphy.
        Prostaglandins and Medicine. 1979; 3: 295
        • Levine L.
        • Moskowitz M.A.
        α- and β-Adrenergic stimulation of arachidonic acid metabolism in cells in culture.
        in: Proceedings National Academy of Science USA. 76. 1979: 6632
        • Lands W.E.B.
        • Samuelsson B.
        Phospholipid precursor of prostaglandins.
        Biochim. Biophys. Acta. 1968; 164: 426
        • Lowry O.H.
        • Rosenbrough N.J.
        • Farr A.I.
        • Randall R.J.
        Protein measurement with the Folin phenol reagent.
        Journal Biological Chemistry. 1951; 193: 265
        • Hostetter T.H.
        • Troy J.L.
        • Brenner B.M.
        Glomerular hemodynamics in experimental diabetes mellitus.
        Kidney International. 1981; 19: 410
        • Hostetter T.H.
        • Olson J.L.
        • Rennke H.G.
        • Venkatachalam M.A.
        • Brenner B.M.
        Hyperfiltration in remnant nephrons: A potentially adverse response to renal ablation.
        American Journal Physiology. 1981; 241: F85

      Article info

      Identification

      DOI: https://doi.org/10.1016/0262-1746(83)90097-5

      Copyright

      © 1983 Published by Elsevier Inc.

      ScienceDirect

      Access this article on ScienceDirect
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX