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Abstract
Previous experiments have suggested that superoxide dismutase (SOD) and catalase (CAT)
may inhibit prostaglandin synthesis. The purpose of this study was to determine if
these free radical scavengers can alter the metabolism of free arachidonic acid (AA)
by the cyclooxygenasse and lipoxygenase enzyme systems in platelets. In control experiments
washed platelets were incubated with 3H-AA for 5 minutes, extracted and the products separated by reverse phase high pressure
liquid chromatography (HPLC). In normal intact platelets 13.5 ± 0.6% of the radioactivity
was found in TxB2, 16.3 ± 1.4% in HHT, 61.3 ± 1.1% in 12-HETE and 9.0 ± 1.0% was unconverted AA. Pre-incubating
the platelets for 1 minute with 10 μg/ml SOD or CAT or 10 μg/ml SOD plus 10 μg/ml
CAT did not inhibit AA conversion or alter the percent product distribution. Similarly,
SOD and CAT had no effect on AA metabolism in broken cells. However, as expected,
pretreating platelets with indomethaoin blocked TxB2,and HHT formation (P <.0001). We conclude that SOD and CAT do not inhibit cyclooxygenase
or lipoxygenase metabolism of free AA in platelets.
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References
- Cerebral arteriolar damage by arachidonic acid and prostaglandin G2.Science. 1980; 209: 1242
- Inhibition by free radical scavengers and by cyclooxygenase inhibitors of pial arteriolar abnormalities from concussive brain injury in cats.Circulation Res. 1981; 48: 95
- Prostaglandin biosynthesis can be triggered by lipid peroxides.Archiv. Biochem. Biophys. 1979; 193: 340
- Isolation and structure of two prostaglandin endoperoxides that cause platelet aggregation.in: 8th ed. Proc. Nat. Acad. Sci., USA. 71. 1974: 345
- Preparation and stability of indomethacin solutions.Can. J. Physiol. 1982; 60: 988
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© 1984 Published by Elsevier Inc.
