Research Article| Volume 13, ISSUE 2, P171-178, February 1984

Albumin stimulates the release of arachidonic acid from phosphatidylcholine in hamster lungs

      This paper is only available as a PDF. To read, Please Download here.


      14C-Arachidonic acid injected into the pulmonary circulation of isolated hamster lungs was effectively incorporated into lung lipids. Once retained the radiolabel was relatively stable but the release of radioactivity increased up to 10-fold when bovine serum albumin (1 %) was added to the perfusate. This efflux of radioactivity was not blocked by quinacrine, a phospholipase A2 inhibitor. In albumin experiments the released 14C-araehidonate griginated mainly from the phospholipid fraction in which phosphatidylcholine was the main source of the released radioactivity.Pulmonary infusion of albumin had no significant effect on the amount of 14C-arachidonic acid in the neutral lipid or free fatty acid fractions of perfused lungs. In experiments with albumin about 80 % of the released radioactivity co-chromatographed with unlabelled arachidonic acid whereas in the absence of albumin only about 20 % of the released radioactivity was unmetabolized arachidonic acid. This study indicates that albumin stimulates the release of arachidonic acid from isolated hamster lungs and that the release is increased mainly from the phosphatidyl choline fraction.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Prostaglandins, Leukotrienes and Essential Fatty Acids
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Isakson P.C.
        • Raz A.
        • Needleman P.
        Selective inoorporation, of 14C-araohidonic acid into the phospholipids and subsequent metabolism to C-prostaglandins.
        Prostaglandins. 1976; 12: 739
        • Al-Ubaidi F.
        • Bakhle Y.S.
        Metabolism of 14C-arachidonate in rat isolated lung.
        Prostaglandins. 1980; 19: 747
        • Isakson P.C.
        • Raz A.
        • Denny S.E.
        • Wyche A.
        Hormonal stimulation of arachidonate release from isolated perfused organs. Relationship to prostaglandin biosynthesis.
        Prostaglandins. 1977; 14: 853
        • Jose P.I.
        • Seale J.P.
        Incorporation and metabolism of (14 C)-arachidonic acid in guinea-pig lungs.
        Br. J. Pharmacol. 1979; 67: 519
        • Skipski V.P.
        • Peterson R.F.
        • Barclay M.
        Quantitative analysis of phospholipids by thin-layer chromatography.
        Biochem. J. 1964; 90: 374
        • Billah M.M.
        • Lapetina E.G.
        • Cuatrecasas P.
        Phospholipase A and phospholipase C activities of platelets.
        J. Biol. Chem. 1979; 255: 10227
        • Uotila P.
        • Hiltunen A.
        • Mäkilä K.
        • Männistö J.
        • Toivonen H.
        • Hartiala J.
        The metabolism of arachidonic acid in isolated hamster, rat and guinea pig lungs.
        Prostaglandins Med. 1980; 5: 149
        • Chap H.
        • Zwaal R.F.A.
        • Van Deenen L.L.M.
        Action of highly purified phospholipases on blood platelets. Evidence for an asymmetric distribution of phospholipids in the surface membrane.
        Biochim. Biophys. Acta. 1977; 467: 146
        • Perret B.
        • Chap H.
        • Douste-Blazy L.
        Asymmetric distribution of arachidonic acid in the plasma membranes of human platelets. A determination using purified phospholipases and a rapid method for membrane isolation.
        Biochim. Biophys. Acta. 1979; 556: 434
        • Dise C.A.
        • Burch J.W.
        • Goodman D.B.P.
        Direct interaction of mepacrine with erythrocyte and platelet membrane phospholipids.
        J.Biol.Chem. 1982; 257: 4701