The effect of oxytocin, estrogen, calcium ionophore A23187 and hydrocortisone on prostaglandin F2α and 6-oxo-prostaglandin F1α production by cultured human endometrial and myometrial explants

H.A. Leaver; D.H. Richmond

doi:10.1016/0262-1746(84)90009-X

Research Article| Volume 13, ISSUE 2, P179-196, February 1984

The effect of oxytocin, estrogen, calcium ionophore A23187 and hydrocortisone on prostaglandin F_2α and 6-oxo-prostaglandin F_1α production by cultured human endometrial and myometrial explants

H.A. Leaver
H.A. Leaver
Correspondence
Reprint request to HAL
Affiliations
Napier College, Colinton Road, Edinburgh EH10 5DT, Scotland
Search for articles by this author
D.H. Richmond
D.H. Richmond
Affiliations
Eastern General Hospital, Edinburgh EH7 LN, Scotland
Search for articles by this author

DOI:https://doi.org/10.1016/0262-1746(84)90009-X

This paper is only available as a PDF. To read, Please Download here.

Abstract

Normal human endometrium (classified by histology and date after last menstrual period) was cultured for 72h, and the output of prostaglandin F₂α and 6-oxo-prostaglandin Fla detected by radioimmunoassay. Hormones/stimuli were added to the culture during the second day of culture for 5h and 19h periods.

1.
1) The output of prostaglandin F₂α from cultured endometrium was significantly higher (p<0.05) at the beginning (d4–8) and end (d25–30) of the menstrual cycle, compared to mid-cycle (d13–24) endometrium. Significantly more prostaglandin F₂α was released from proliferative than from secretory phase endometrium (p<0.02).
2.
2) Prostaglandin F₂α release was rapidly stimulated by sodium arachidonate (20–300 μg/ml), and by calcium ionophore A23187 (5 μg/ml) at an extracellular calcium ion concentration of 1.8mM.The ionophore stimulation was greater in mid-cycle endometrium than in endometrium from the beginning or the end of the menstrual cycle.
3.
3) Estradiol-17β (10 ng/ml) gradually increased the output of prostaglandin F₂α from secretory phase endometrium, and this stimulation was observed in the post-incubation period after hormone had been removed from the incubation medium.
4.
4) Oxytocin (1 × 10⁻⁵U/ml caused a more rapid stimulation of prostaglandin F₂α output from secretory phase tissue (p<0.05 during the first 5h incubation period with hormone).
5.
5) Oxytocin (1 × 10⁻⁵ U/ml) and estradiol (long/ml) together significantly stimulated prostaglandin F2a production by proliferative as well as secretory phase endometria.
6.
6) A high dose of hydrocortisone (loo μg/ml) inhibited the output of prostaglandin F₂α from proliferative and secretory phase endometrium and also from ionophore-stimulated endometrium. However, this dose of hydrocortisone did not inhibit the synthesis of prostaglandin F2a from exogenous arachidonic acid, or the estradiol-induced increase in prostaglandin F₂α production.
7.
7) Co-culture of endometrium with myometrium did not modify the output of prostaglandin F₂α or of 6-oxo-prostaglandin Fla from cultured tissues.
8.
8) These experiments suggest that arachidonic acid supply to the cyclooxygenase enzyme may vary during the menstrual cycle: and indicate a gradual increase in prostaglandin synthesising capacity in response to estrogen, more rapid control via oxytocin, and an interaction between estrogen and oxytocin to modulate prostaglandin F2a synthesis in human endometrium.

To read this article in full you will need to make a payment

Purchase one-time access:

One-time access price info

For academic or personal research use, select 'Academic and Personal'
For corporate R&D use, select 'Corporate R&D Professionals'

Subscribe:

Already a print subscriber? Claim online access

Already an online subscriber? Sign in

Register: Create an account

Institutional Access: Sign in to ScienceDirect

References

- Gréen K.
Determinations of prostaglandins in body fluids and tissues.
Acta. Obsetrica Gynecologica Scandanavica. 1979; 87 (Suppl.): 5-20
- Google Scholar
- Willman E.A.
- Collins W.P.
- Clayton S.G.
Studies on involvement of prostaglandins in uterine symptomatology and pathology.
British Journal Obstetrics & Gynaecology. 1976; 83: 337-341
- Google Scholar
- Ham E.A.
- Cirillo V.J.
- Zanetti M.E.
- Kuel F.A.
Estrogen-directed synthesis of specific prostaglandins by the uterus.
in: Proc. Nat. Acad. Sci.72. 1975: 1420-1424
- Google Scholar
- Thaler Dao H.
- Ramonatxo M.
- Saintot M.
- Chaintreuil J.
- Crastes de Paulet A.
Effect of oestradiol 175 on prostaglandin biosynthesis by the uterus of ovariectomised rat and guinea pig.
Prostaglandins. 1982; 24: 149-163
- Google Scholar
- Lewis T.M.
- Parry D.M.
- Robinson J.S.
- Thorburn G.D.
- Challis J.R.-G.
Effect of progesterone and estrogen on prostaglandin F2αnd 13-14-dihydro-15-oxo-prostaglandin F_1α in uteri and plasma of ovariectomised ewes.
J. Endocrinol. 1977; 73: 427-439
- Google Scholar
- Dey S.K.
- Hoversland R.C.
- Johnson D.C.
Phospholipase A2 activity in the rat uterus: modulation by steroid hormones.
Prostaglandins. 1982; 23: 619-630
- Google Scholar
- Batra S.
- Bengtsson B.
Effect of diethylstilbestrol and ovarian steroids on contractile responses and calcium movements in rat uterine smooth muscle.
J. Physiology. 1978; 276: 329-342
- Google Scholar
- Garfield R.E.
- Kannan M.S.
- Daniel E.E.
Gap junction formation in the myometrium.
Amer. J. Physiol. 1980; 238: C81-89
- Google Scholar
- Roberts J.S.
- McCracken J.A.
- Gavagan J.E.
- Soloff M.S.
Oxytocin stimulated release of prostaglandin F₂α from ovine endometrium in vitro.
Endocrinology. 1976; 99: 1107-1114
- Google Scholar
- Leaver H.A.
- Seawright A.
Control of endometrial prostaglandin output in vitro during the estrous cycle of the guinea-pig: influence of estradiol 17-β, progesterone, oxytocin and calcium ionophore A23187.
Prostaglandins, Leucotrienes & Medicine. 1982; 9: 657-670
- Google Scholar
- Soloff M.S.
Regulation of oxytocin action at the receptor level Life Sciences. 1979; 25: 1453-1460
- Google Scholar
- Michell R.H.
Inositol lipids and cell surface receptor function.
Biochimica Biophysica Acta. 1975; 415: 81-147
- Google Scholar
- Irvine R.F.
How is the level of free arachidonic acid controlled in mammalian cells?.
Biochemical Journal. 1982; 204: 3-16
- Google Scholar
- Hirata F.
- Notsu Y.
- Iwata M.
- Parente L.
- Dirosa M.
- Flower R.J.
Identification of several species of phospholipase inhibitory proteins by radicimmunaossay for lipomodulin.
Biochemical Biophysical Research Communications. 1982; 109: 223-230
- Google Scholar
- Abel M.
- Kelly R.W.
Differential production of PGS within the human uterus.
Prostaglandins. 1979; 18: 821-828
- Google Scholar
- Baker T.G.
- Neal P.
Effects of x-irradiation on mammalian oocytes in organ culture.
Biophysik. 1969; 6: 39-43
- Google Scholar
- Leaver H.A.
- Ning A.C.W.S.
Endometrial uptake and esterification of arachidonic acid during the cestrous cycle of the guinea-pig.
British Journal of Pharmacology. 1981; 73: 223p
- Google Scholar
- Liggins G.C.
- Campos G.A.
- Roberts C.M.
- Skinner S.J.
Production rates of PGF, 6-keto-PGF_1α and thromboxane B2 by perfused human endometrium.
Prostaglandins. 1980; 19: 461
- Google Scholar
- Leavitt M.J.
- Tobon H.
- Josimovich J.B.
PG Content of human endometrium.
Fertility & Sterility. 1975; 26: 296-305
- Google Scholar
- Maathius J.B.
- Kelly K.W.
Concentrations of PGF_2α and E₂ in endometrium throughout the human menstrual cycle.
J. Endocrinology. 1978; 77: 361-371
- Google Scholar
- Schwartzman M.
- Lieberman E.
- Raz A.
Bradykinin and angiotensin activation of arachidonic acid deacylation in rabbit kidney. Hormone-sensitive versus hormone-insensitive pools and arachidonic acid.
J. Biological Chemistry. 1981; 256: 2329-2333
- Google Scholar
- Mitchell S.
- Poyser N.H.
- Wilson N.H.
Effect of p-bromo-phenacylbromide on arachidonic acid and prostaglandin synthesis by guinea-pig uterus in vitro.
British J. Pharmacology. 1977; 59: 107-113
- Google Scholar
- Leaver H.A.
- Poyser N.H.
Distribution of arachidonic acid and other fatty acids in lipids of guinea-pig uterus and plasma in relation to uterine prostaglandin synthesis.
J. Reproduction and Fertility. 1981; 61: 325-333
- Google Scholar
- Leaver H.A.
Calcium sensitive prostaglandin output and phospholipase activity in human and guinea-pig endometrium.
in: Proceedings International Protaglandin Conference. 5. 1982: 394-395
- Google Scholar
- Fang B.K.
- Ooi T.C.
Prostaglandin secretion by human endometrium in vitro.
American J. Obstetrics & Gynecology. 1982; 142: 626-633
- Google Scholar
- Aizawa Y.
- Mueller G.C.
The Effect in vivo and in vitro of estrogens on lipid synthesis in rat uterus.
J. Biological Chemistry. 1961; 236: 381-386
- Google Scholar
- Spooner P.M.
- Gorski J.
Early estrogen effects on lipid metabolism in rat uterus.
Endocrinology. 1972; 91: 1273-1283
- Google Scholar
- Castracane V.D.
- Jordan V.C.
Considerations into mechanisms of estrogen-stimulated prostaglandin synthesis.
Prostaglandins. 1976; 12: 243-251
- Google Scholar
- Husslein P.
- Fuchs A.R.
- Fuchs F.
Oxytocin and initiation of human parturitian: prostaglandin release during induction of labour by oxytocin.
American J. Obstetrics & Gynecology. 1981; 141: 688-691
- Google Scholar
- Fuchs A.R.
- Husslein P.
- Fuchs F.
Oxytocin and initiation of human parturition:stimulation of decidual prostaglandin production.
American J. Obstetrics & Gynecology. 1982; 141: 694-699
- Google Scholar
- Wathes D.C.
- Swann R.W.
Is oxytocin an ovarian hormone?.
Nature. 1982; 297: 225-227
- Google Scholar

Article info

Identification

DOI: https://doi.org/10.1016/0262-1746(84)90009-X

Copyright

ScienceDirect

Access this article on ScienceDirect

Property	Value
Status
Version
Ad File
Disable Ads Flag
Environment
Moat Init
Moat Ready
Contextual Ready
Contextual URL
Contextual Initial Segments
Contextual Used Segments
AdUnit
SubAdUnit
Custom Targeting
Ad Events
Invalid Ad Sizes