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Abstract
We examined the interactions between supernatant from FMLP-activated human granulocytes,
recombinant interleukin-1 (IL-1) and recombinant tumor necrosis factor (TNF) in the
stimulation of prostaglandin E2 (PGE2) production by human amnion cells. Amnion cells from elective term cesarian sections
were cultured in monolayer culture. Human granulocytes were activated with FMLP and
centrifuged to obtain cell-free supernatant. Amnion cells were treated with granulocyte
supernatant, IL-1α, IL-1β, TNF-α, TNF-β, or different combinations of these. Each
of the stimulators alone enhanced the PGE2 production 5-to 27-fold. Granulocyte supernatant was synergistic with each of the
cytokines. The combinations of IL-1α or IL-1β with either TNF-α or TNF-β caused a
synergistic stimulation of amnion cell PGE2 production as well, whereas the combinations of IL-1α with IL-1β or of TNF-β with
TNF-β were not synergistic. Furthermore, granulocyte supernatant was synergistic with
the combination of IL-1 and TNF, resulting in a more than 150-fold stimulation of
PGE2 production. Indomethacin completely suppressed these effects. We propose that granulocyte
products acting together with IL-1 and TNF enhance PGE2 synthesis during inflammation, and serve as signals for the initiation of preterm
labor in the setting of intra-amniotic infection.
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Article info
Publication history
Accepted:
July 3,
1991
Received:
April 2,
1991
Identification
Copyright
© 1991 Published by Elsevier Inc.
