Advertisement

Sperm fatty acid composition in subfertile men

Published:August 10, 2006DOI:https://doi.org/10.1016/j.plefa.2006.06.002

      Abstract

      Introduction

      The lipid composition of spermatozoa plays an important role for successful fertilization.

      Patients and methods

      In the present study, we analyzed the fatty acid (FA) composition of spermatozoa of normozoospermic, asthenozoospermic, oligozoospermic and oligoasthenozoospermic men.

      Results

      Spermatozoa from asthenozoospermic (P<0.01), oligozoospermic (P<0.05) and oligoasthenozoospermic men (P<0.05) had lower levels of docosahexaenoic acid (22:6w3, DHA) than those from normozoospermic men. In oligozoospermic and asthenozoospermic men, spermatozoa 18:0 content was higher than that of normozoospermics (P<0.01 and P<0.001, respectively). 18:1w9 was higher in oligoasthenozoospermic and oligozoospermic samples when compared with normozoospermic samples (P<0.05 for both). While from the point of view of total w6 FAs there was no significant difference among the groups, the w6/w3 ratio was significantly higher in asthenozoospermic samples than in normozoospermic samples (P<0.05). Monounsaturated fatty acids (MFA) were higher in oligozoospermic samples (P<0.05) than in normozoospermic samples, polyunsaturated fatty acids (PUFA) were lower in asthenozoospermic (P<0.01), oligoasthenozoospermic (P<0.05) and oligozoospermic samples (P<0.05) than in normozoospermic samples.
      Saturated fatty acids (SFA) were significantly higher in asthenozoospermic (P<0.01) and oligozoospermic samples (P<0.05) compared with normozoospermic samples. In correlation analysis, there were significant positive correlations between DHA with sperm motility ( r = 0.53 ), sperm concentration ( r = 0.36 ) and normal sperm morphology ( r = 0.30 ). In addition, there were significant correlations between PUFA with sperm motility ( r = 0.50 ), sperm concentration ( r = 0.35 ), and normal sperm morphology ( r = 0.28 ), and between w6/w3 with sperm motility ( r = - 0.47 ), sperm concentration ( r = - 0.27 ), and normal sperm morphology( r = - 0.24 ).

      Discussion

      These suggest that decreased DHA and PUFA, and increased w6/w3 in spermatozoa may be related to infertility in oligo- and/or asthenozoospermic men.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Prostaglandins, Leukotrienes and Essential Fatty Acids
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Miller Jr., R.R.
        • Sheffer C.J.
        • Cornett C.L.
        • McClean R.
        • MacCallum C.
        • Johnston S.D.
        Sperm membrane fatty acid composition in the Eastern grey kangaroo (Macropus giganteus), koala (Phascolarctos cinereus), and common wombat (Vombatus ursinus) and its relationship to cold shock injury and cryopreservation success.
        Cryobiology. 2004; 49: 137-148
        • Ahluwalia B.
        • Holman R.T.
        Fatty acid composition of lipids of bull, boar, rabbit and human semen..
        J Reprod Fertil. 1969; 18: 431-437
        • Zalata A.A.
        • Christophe A.B.
        • Depuydt E.
        • Schonjans F.
        • Comhaire F.H.
        The fatty acid composition of phospholipids of spermatozoa from infertile patients.
        Mol Hum Reprod. 1998; 4: 111-118
        • Conquer J.A.
        • Martin J.B.
        • Tummon I.
        • Watson L.
        • Tekpetey F.
        Fatty acid analysis of blood serum, seminal plasma, and spermatozoa of normozoospermic vs. asthenozoospermic males.
        Lipids. 1999; 34: 793-799
        • Calamera J.
        • Buffone M.
        • Ollero M.
        • Alvarez J.
        • Doncel G.F.
        Superoxide dismutase content and fatty acid composition in subsets of human spermatozoa from normozoospermic, asthenozoospermic, and polyzoospermic semen samples.
        Mol Reprod Dev. 2003; 66: 422-430
        • Nissen H.P.
        • Kreysel H.W.
        Polyunsaturated fatty acids in relation to sperm motility.
        Andrologia. 1983; 15: 264-269
        • Gulaya N.M.
        • Melnik A.A.
        • Balkov D.I.
        • Volkov G.L.
        • Vysotskiy M.V.
        • Vaskovsky V.E.
        The effect of long-chain N-acylethanolamines on some membrane-associated functions of neuroblastoma C1300 N18 cells.
        Biochim Biophys Acta. 1993; 1152: 280-288
        • Connor W.E.
        • Lin D.S.
        • Wolf D.P.
        • Alexander M.
        Uneven distribution of desmosterol and docosahexaenoic acid in the heads and tails of monkey sperm.
        J Lipid Res. 1998; 39: 1404-1411
        • Hartree E.
        • Mann T.
        Plasmalogen in ram sperm, and its role in sperm metabolism.
        Biochem J. 1961; 80: 464-471
        • World Health Organization
        Who Laboratory Manual for the Examination of Human Semen-servical Mucus Interaction.
        Cambridge University Press, Cambridge1999 (pp.4–23)
        • Tappel A.L.
        Measurement and protection from in vivo lipid peroxidation.
        in: Caughey W.S. Biochemical and Clinical Aspects of Oxygen. Academic Press, New York1979: 679-698
        • Zalata A.A.
        • Christophe A.B.
        • Depuydt C.E.
        • Schoonjans F.
        • Comhaire F.H.
        White blood cells cause oxidative damage to the fatty acid composition of phospholipids of human spermatozoa.
        Int J Androl. 1998; 21: 154-162
        • Nakamura H.
        • Kimura T.
        • Nakajima A.
        • Shimoya K.
        • Takemura M.
        • Hashimoto K.
        • Isaka S.
        • Azuma C.
        • Koyama M.
        • Murata Y.
        Detection of oxidative stress in seminal plasma and fractionated sperm from subfertile male patients.
        Eur J Obstet Gynecol Reprod Biol. 2002; 105: 155-160
        • Suleiman S.A.
        • Ali M.E.
        • Zaki Z.M.
        • el-Malik E.M.
        • Nasr M.A.
        Lipid peroxidation and human sperm motility: Protective role of vitamin E.
        J Androl. 1996; 17: 530-537
        • Tavilani H.
        • Doosti M.
        • Saeidi H.
        Malondialdehyde levels in sperm and seminal plasma of asthenozoospermic and its relationship with semen parameters.
        Clin Chim Acta. 2005; 356: 199-203
        • Müller K.
        • Pomorski T.
        • Müller P.
        • Herrmann A.
        Stability of transbilayer phospholipid asymmetry in viable ram sperm cells after cryotreatment.
        J Cell Sci. 1999; : 11-20
        • Lenzi A.
        • Gandini L.
        • Picardo M.
        • Tramer F.
        • Sandri G.
        • Panfili E.
        Lipoperoxidation damage of spermatozoa polyunsaturated fatty acids (PUFA): scavenger mechanisms and possible scavenger therapies.
        Front Biosci. 2000; 5: 1-15
        • Flesch F.M.
        • Gadella B.M.
        Dynamics of the mammalia sperm plasma membrane in the process of fertilization.
        Biochim Biophys Acta. 2000; 1469: 197-235
        • Wolf D.E.
        Diffusion and control of membrane regionalization.
        Ann NY Acad Sci. 1987; 513: 247-261
        • Stubbs C.D.
        • Smith A.D.
        The modification of the mammalian membrane of polyunsaturated fatty acid composition in relation to membrane fluidity and function.
        Biochem Biophys Acta. 1984; 779: 89-137
        • Aveldano M.
        • Rotstein N.
        • Vermouth N.
        Lipids remodelling during epididymal maturation of rat spermatozoa. Enrichment in plasmenylcholines containing long-chain polyenoic fatty acids of the n-9 series.
        Biochem J. 1992; 283: 235-421
        • Zaniboni L.
        • Rizzi R.
        • Cerolini S.
        Combined effect of DHA and alpha-tocopherol enrichment on sperm quality and fertility in the turkey.
        Theriogenology. 2005; 17 ([Epub ahead of print])
        • Brinsko S.P.
        • Varner D.D.
        • Love C.C.
        • Blanchard T.L.
        • Day B.C.
        • Wilson M.E.
        Effect of feeding a DHA-enriched nutriceutical on the quality of fresh, cooled and frozen stallion semen.
        Theriogenology. 2005; 63: 1519-1527