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Effect of Chia oil (Salvia Hispanica) rich in ω-3 fatty acids on the eicosanoid release, apoptosis and T-lymphocyte tumor infiltration in a murine mammary gland adenocarcinoma

  • C.E. Espada
    Affiliations
    Iª Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Ciudad Universitaria. Casilla de Correos 220, 5000, Córdoba. Argentina
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  • M.A. Berra
    Affiliations
    Iª Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Ciudad Universitaria. Casilla de Correos 220, 5000, Córdoba. Argentina
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  • M.J. Martinez
    Affiliations
    Laboratorio de Calidad de Granos. EEA-INTA Manfredi. Ruta Nac. 9km 636. Córdoba, Argentina
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  • A.R. Eynard
    Affiliations
    Iª Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Ciudad Universitaria. Casilla de Correos 220, 5000, Córdoba. Argentina
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  • M.E. Pasqualini
    Correspondence
    Corresponding author. Tel.: +543514334020; fax: +543514810404.
    Affiliations
    Iª Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Ciudad Universitaria. Casilla de Correos 220, 5000, Córdoba. Argentina
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      Abstract

      We investigated the effects of certain dietary polyunsaturated fatty acids (PUFAs) and related eicosanoids on the growth and metastasis formation of a murine mammary gland adenocarcinoma. Salvia hispanica (ChO) and Carthamus tinctorius (SaO) vegetable oil sources of ω-3 and -6 PUFAs and a commercial diet as control (CO), were used. We analysed fatty acids of neoplastic cells (NC) membranes by GLC; the eicosanoids 12- HETE and 12-HHT (LOX and COX metabolites) by HPLC and apoptosis and T-lymphocyte infiltration by flow cytometry and microscopy. NC from ChO groups showed lower levels of arachidonic acid and of both eicosanoids compared to SaO and CO (p<0.05). The ChO diet decreased the tumor weight and metastasis number (p<0.05). Apoptosis and T-lymphocyte infiltration were higher and mitosis decreased with respect to the other diets (p<0.05). Present data showed that ChO, an ancient and almost unknown source of ω-3, inhibits growth and metastasis in this tumor model.
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