Abstract
We investigated the effects of certain dietary polyunsaturated fatty acids (PUFAs)
and related eicosanoids on the growth and metastasis formation of a murine mammary
gland adenocarcinoma. Salvia hispanica (ChO) and Carthamus tinctorius (SaO) vegetable oil sources of ω-3 and -6 PUFAs and a commercial diet as control (CO), were used. We analysed fatty
acids of neoplastic cells (NC) membranes by GLC; the eicosanoids 12- HETE and 12-HHT
(LOX and COX metabolites) by HPLC and apoptosis and T-lymphocyte infiltration by flow cytometry and microscopy. NC from ChO groups showed
lower levels of arachidonic acid and of both eicosanoids compared to SaO and CO (p<0.05). The ChO diet decreased the tumor weight and metastasis number (p<0.05). Apoptosis and T-lymphocyte infiltration were higher and mitosis decreased with respect to the other
diets (p<0.05). Present data showed that ChO, an ancient and almost unknown source of ω-3, inhibits growth and metastasis in this tumor model.
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References
- Potential of essential fatty acids as natural therapeutic products for human tumors.Nutrition. 2003; 19: 386-388
- Essential fatty acids: biochemistry, physiology and pathology.Biotechnol. J. 2006; 1: 420-439
L. Dajuel, H.H. Mündel, Safflower: Carthamus tinctorius, International Plant Genetic Resources Institute, 1996, pp: 1–83.
- Differential effects of dietary Oenothera, Zizypus mistol and Corn oils and essential fatty acid deficiency on the progression of a murine mammary gland adenocarcinoma.Nutrition. 1999; 15: 208-212
- Chia seed (Salvia hispanica L.) as an ω-3 fatty acid source for broilers: influence on fatty acid composition, cholesterol and fat content of white and dark meats, growth performance, and sensory characteristics.Poult. Sci. 2002; 81: 826-837
- Polyunsaturated fatty acids (PUFA) and eicosanoids in human health and pathologies.Biomed. Pharmacot. 2000; 56: 215-222
- The mechanisms of lipoxygenase inhibitor-induced apoptosis in human breast cancer cells.Biochem. Biophys. Res. Commun. 2002; 296: 942-948
- 12-lipoxygenase metabolite 12(S)-HETE stimulates human pancreatic cancer cell proliferation via protein tyrosine phosphorylation and ERK activation.Int. J. Cancer. 2001; 94: 630-636
- Mechanisms regulating tumor angiogenesis by 12-lipoxygenase in prostate cancer cells.J. Biol. Chem. 2006; 281: 18601-18609
- Xenobiotic metabolizing cytochromes P450 convert prostaglandin endoperoxide to hydroxyheptadecatrienoic acid and mutagen, malondialdehyde.J. Biol. Chem. 2000; 275: 11784-11790
- Regulation of apoptosis through arachidonate cascade in mammalian cells.Appl. Biochem. Biotech. 2002; 102–103: 239-250
- Molecular mechanisms of action and health benefits of polyunsaturated fatty acids.Rev. Invest. Clin. 2005; 57: 457-472
- Pivotal role of PGE2 and IL-10 in the cross-regulation of dendritic cell-derived inflammatory mediators.Cell Mol. Immunol. 2006; 3: 271-277
- Regulation of calcium signaling by docosahexaenoic acid in human T-cells: implication of CRAC channels.J. Lipid. Res. 2000; 41: 277-284
- N-3 polyunsaturated fatty acid supplementation alters inositol phosphate metabolism and protein kinase C activity in adult porcine cardiac myocytes.J. Nutr. Biochem. 2001; 12: 7-13
- Comparative effects of eicosapentaenoic acid and docosahexaenoic acid on proliferation, cytokine production and pleiotropic gene expression in Jurkat cells.J. Nutr. Biochem. 2004; 15: 657-665
- Modulation in vitro of human natural cytotoxicity, lymphocyte proliferative response to mitogens and cytokine production by essential fatty acids.Immunology. 1997; 92: 166-172
- Dietary lipids, eicosanoid release and apoptosis of cells of a murine lung alveolar carcinoma.Prost. Leuk Essent Fatty Acids. 2005; 72: 235-240
- Characterization of two murine mammary adenocarcinoma tumors with different metastatic ability.J. Exp. Clin. Cancer Res. 1983; 2: 51-160
- Fatty acids specifically related to the anisotropic properties of plasma membrane for rat urothelium.Biochim. Biophys. Acta. 2000; 1483: 174-184
- COX and LOX eicosanoids modulate platelet activation and procoagulation induced by two murine cancer cells.Prost. Leuk Essent Fatty Acids. 2000; 63: 377-383
- Association between E-catherin expression by human colon, bladder and breast cancer cells and the 13-HODE: 15-HETE ratio, a possible role of their metastatic potential.Prost. Leuk Essent Fatty Acids. 2003; 68: 9-16
- A novel assay for apoptosis: flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V.J. Immunol. Methods. 1995; 180: 38-52
- Morphological, biochemical and flow cytometric assays of apoptosis.in: Coligan J. Kruisbeek A.M. Margulies D. Shevach E.M. Strober W. Current Protocols in Immunology. Wiley, New York, NY1995: 3.17.1-3.17.33
- Valuation of exfoliative cytology as prediction factor in oral mucosa lesions.Oral. Med. Pathol. 2005; 10: 92-102
- Dietary fats and membrane function: implications for metabolism and disease.Biol. Rev. 2005; 80: 155-169
- Dietary long-chain n-3 fatty acids for prevention of cancer: a review of potential mechanisms.Am. J. Clin. Nutr. 2004; 79: 935-945
- CD8+ lymphocyte infiltration and apoptosis in hepatocellular carcinoma.Eur. J. Surg. Oncol. 2004; 30: 53-57
- Mechanisms of omega-3 fatty acid-induced growth inhibition in MDA-MB-231 human breast cancer cells.Breast Cancer Res. Treat. 2005; 92: 187-195
- Increased metastatic potential in human prostate carcinoma cells by overexpression of arachidonate 12-lipoxygenase.Clin. Exp. Metast. 2003; 20: 657-663
- Decreased n-6/n-3 fatty acid ratio reduces the invasive potential of human lung cancer cells by downregulating of cell adhesion/invasion-related genes.Carcinogenesis. 2005; 26: 779-784
Article info
Publication history
Published online: July 09, 2007
Accepted:
May 22,
2007
Received in revised form:
May 11,
2007
Received:
February 16,
2007
Footnotes
☆This work was supported by Grants from CONICET, FONCYT, AGENCIA CÓRDOBA CIENCIA, SECYT and Fundación para el Progreso de la Medicina de la Ciudad de Córdoba (Argentina).
Identification
Copyright
© 2007 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
