Abstract
Dietary n-3PUFA and gut bacteria, particularly Bacteroidetes, have been suggested to be related to adiposity. We investigated if n-3PUFA affected fat storage and cecal bacteria in piglets. Twenty-four 4-day-old piglets
were allocated to formula rich in n-3PUFA (∼3E%) from fish oil (FO) or n-6PUFA from sunflower oil (SO) for 14 days. We assessed body weight, fat accumulation
by dual-energy X-ray absorptiometry and microbial molecular fingerprints. Dietary
PUFA-composition was reflected in higher erythrocyte n-3PUFA in the FO- than the SO-group (P<0.001). Principal component analysis revealed group differences in the overall microbiotic
composition, which involved a larger Bacteroides community in the SO-group (P=0.02). There was no significant difference in body fat percentage and no relationship
between fat accumulation and gut Bacteroides. Hence, this study does not support an impact of n-3PUFA or microbiota on fat accumulation during the postnatal maturation period. The
impact of dietary PUFA on the gut Bacteroides warrants further investigation.
Abbreviations:
DEXA (dual-energy X-ray absorptiometry), FO (fish oil), LCPUFA (long-chain PUFA), MUFA (monounsaturated fatty acids), PCA (principal component analysis), RBC (erythrocytes), SFA (saturated fatty acids), SO (sunflower oil), T-RF (terminal-restriction fragment)Keywords
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References
- Obesity alters gut microbial ecology.Proceedings of the National Academy of Sciences. 2005; 102: 11070-11075
- Microbial ecology: human gut microbes associated with obesity.Nature. 2006; 444: 1022-1023
- An obesity-associated gut microbiome with increased capacity for energy harvest.Nature. 2006; 444: 1027-1131
P.J. Turnbaugh, M. Hamady, T. Yatsunenko et al. A core gut microbiome in obese and lean twins, Nature, (2008).
- Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs.Letters in Applied Microbiology. 2008; 47: 367-373
- Comparative Study of Bacterial Groups within the Human Cecal and Fecal Microbiota.Applied and Environmental Microbiology. 2001; 67: 4939-4942
- Impact of diet on the intestinal microbiota in 10-month-old infants.Journal of Pediatric Gastroenterology and Nutrition. 2007; 44: 613-618
- Coordinate induction of peroxisomal acyl-CoA oxidase and UCP-3 by dietary fish oil: a mechanism for decreased body fat deposition.Prostaglandins, Leukotrienes and Essential Fatty Acids. 1999; 60: 351-356
- Reduction of adiposity in C57BL/6J mice by dietary omega-3 polyunsaturated fatty acids of marine origin.Diabetologia. 2004; 47: 662
- Eicosapentaenoic acid actions on adiposity and insulin resistance in control and high-fat-fed rats: role of apoptosis, adiponectin and tumour necrosis factor-alpha.British Journal of Nutrition. 2007; 97: 389-398
- Dietary fish oil upregulates intestinal lipid metabolism and reduces body weight gain in C57BL/6J mice.Journal of Nutrition. 2007; 137: 2629-2634
- An emerging risk factor for obesity: does disequilibrium of polyunsaturated fatty acid metabolism contribute to excessive adipose tissue development?.British Journal of Nutrition. 2008; 100: 461-470
- Plasma n-3 polyunsaturated fatty acids are negatively associated with obesity.British Journal of Nutrition. 2009; 102: 1370-1374
- Composition of porcine milk.in: Xu R.J. Cranwell P.D. The Neonatal Pig. Nottingham University Press, 2003 (pp. 213–247)
- Evaluation of dual-energy X-ray absorptiometry for body-composition assessment in piglets and term human neonates.American Journal of Clinical Nutrition. 1996; 63: 157-163
- Maternal fish oil supplementation in lactation: effect on visual acuity and n-3 fatty acid content of infant erythrocytes.Lipids. 2004; 39: 195-206
- A simple method for the isolation and purification of total lipides from animal tissues.Journal of Biological Chemistry. 1957; 226: 497-509
- Preparation of milk fat methyl esters by alcoholysis in an essentially nonalcoholic solution.Journal of Dairy Science. 1969; 52: 1289-1290
- Increased amount of Bifidobacterium thermacidophilum and Megasphaera elsdenii in the colonic microbiota of pigs fed a swine dysentery preventive diet containing chicory roots and sweet lupine.Journal of Applied Microbiology. 2007; 103: 1853-1867
- Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces.Applied and Environmental Microbiology. 2002; 68: 5445-5451
- MiCA: a web-based tool for the analysis of microbial communities based on terminal-restriction fragment length polymorphisms of 16S and 18S rRNA genes.Microbial Ecology. 2007; 53: 562-570
- Impact of polyunsaturated fatty-acids on human colonic bacterial metabolism—an in-vitro and in-vivo study.British Journal of Nutrition. 1995; 74: 733-741
- Cecal environment and TBARS level in mice fed corn oil, beef tallow and menhaden fish oil.Journal of Nutritional Science and Vitaminology. 2000; 46: 65-70
- The effect of dietary oils on cecal microflora in experimental colitis in mice.Indian Journal of Gastroenterology. 2008; 27: 186-189
- The influence of omega-3 polyunsaturated fatty acids (omega-3 pufa) on lactobacilli adhesion to the intestinal mucosa and on immunity in gnotobiotic piglets.Berl Munch Tierarztl Wochenschr. 2003; 116 (July–August): 312-316
- The effect of dietary fatty acids on lactic acid bacteria associated with the epithelial mucosa and from faecalia of Arctic charr, Salvelinus alpinus (L.).Journal of Applied Microbiology. 1998; 85: 855-864
- Dietary omega-3 polyunsaturated fatty acid does not influence the intestinal microbial communities of broiler chickens.Poultry Science. 2009; 88: 2399-2405
- Fish-oil in a high lard diet prevents obesity, hyperlipemia, and adipocyte insulin-resistance in rats.Annals of the New York Academy of Sciences. 1993; 683: 98-101
- Fish-oil n-3 fatty-acids selectively limit the hypertrophy of abdominal fat depots in growing rats fed high-fat diets.American Journal of Physiology. 1993; 264: R1111-R1118
- Dietary n-3 fatty acids affect mRNA level of brown adipose tissue uncoupling protein 1, and white adipose tissue leptin and glucose transporter 4 in the rat.British Journal of Nutrition. 2000; 84: 175-184
- Dietary fish oils limit adipose-tissue hypertrophy in rats.Metabolism—Clinical and Experimental. 1990; 39: 217-219
- Increased uncoupling protein2 mRNA in white adipose tissue, and decrease in leptin, visceral fat, blood glucose, and cholesterol in KK-A(y) mice fed with eicosapentaenoic and docosahexaenoic acids in addition to linolenic acid.Biochemical and Biophysical Research Communications. 1999; 259: 85-90
- Role of dietary fat type in the development of adiposity from dietary obesity-susceptible Sprague?Dawley rats.British Journal of Nutrition. 2003; 89: 429-437
- Prevention of high-fat diet-induced adipose tissue remodeling in obese diabetic mice by n-3 polyunsaturated fatty acids.International Journal of Obesity. 2007; 31: 1004-1013
- Role of fat amount and type in ameliorating diet-induced obesity: insights at the level of hypothalamic arcuate nucleus leptin receptor, neuropeptide Y and pro-opiomelanocortin mRNA expression.Diabetes Obesity & Metabolism. 2004; 6: 35-44
- Adipose tissue inflammation induced by high-fat diet in obese diabetic mice is prevented by n-3 polyunsaturated fatty acids.Diabetologia. 2006; 49: 2109-2119
- Dietary n-3-polyunsaturated fatty acids and energy balance in overweight or moderately obese men and women: a randomized controlled trial.Nutrition and Metabolism. 2009; 6
- Additive benefits of long-chain n-3 polyunsaturated fatty acids and weight-loss in the management of cardiovascular disease risk in overweight hyperinsulinaemic women.International Journal of Obesity. 2006; 30: 1535-1544
- Treatment for 2 mo with n-3 polyunsaturated fatty acids reduces adiposity and some atherogenic factors but does not improve insulin sensitivity in women with type 2 diabetes: a randomized controlled study.American Journal of Clinical Nutrition. 2007; 86: 1670-1679
- Combining fish-oil supplements with regular aerobic exercise improves body composition and cardiovascular disease risk factors.American Journal of Clinical Nutrition. 2007; 85: 1267-1274
- Marine n-3 fatty acids promote size reduction of visceral adipose depots, without altering body weight and composition, in male Wistar rats fed a high-fat diet.British Journal of Nutrition. 2009; 102: 995-1006
- Perilla oil prevents the excessive growth of visceral adipose tissue in rats by down-regulating adipocyte differentiation.Journal of Nutrition. 1997; 127: 1752-1757
- Similar effects on infants of n-3 and n-6 fatty acids supplementation to pregnant and lactating women.Pediatrics. 2001; 108: e82
- Diversity of the human intestinal microbial flora.Science. 2005; 308: 1635-1638
- Evolution of mammals and their gut microbes.Science. 2008; 320: 1647-1651
- Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited.Applied and Environmental Microbiology. 2002; 68: 673-690
- Real-time PCR quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs.Anaerobe. 2008; 14: 224-228
- Human colonic microbiota associated with diet, obesity and weight loss.International Journal of Obesity. 2008; 32: 1720-1724
- What do we mean when we refer to Bacteroidetes populations in the human gastrointestinal microbiota?.Fems Microbiology Letters. 2009; 299: 175-183
- Growth regulation with particular reference to the pig.in: Hollis G.R. Growth of the Pig. CAB International, 1993: 1-32
- Conjugated linoleic acid reduces body fat accretion and lipogenic gene expression in, neonatal pigs fed low- or high-fat formulas.Journal of Nutrition. 2008; 138: 449-454
- Muscle cellularity and postnatal-growth in the pig.Journal of Animal Science. 1993; 71: 3339-3343
- The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing.Plos Biology. 2008; 6: 2383-2400
Article info
Publication history
Published online: August 31, 2011
Accepted:
August 12,
2011
Received in revised form:
August 11,
2011
Received:
June 7,
2011
Footnotes
☆Financial support: This study was supported by The Danish Council for Strategic Research, Program Commission for Food and Health and the intervention oils were donated by Axellus A/S (Oslo, Norway).
Identification
Copyright
© 2011 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
