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A diet high in α-linolenic acid and monounsaturated fatty acids attenuates hepatic steatosis and alters hepatic phospholipid fatty acid profile in diet-induced obese rats

  • Danielle Hanke
    Affiliations
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada

    Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB, Canada
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  • Peter Zahradka
    Affiliations
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada

    Department of Physiology, University of Manitoba, Winnipeg, MB, Canada

    Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB, Canada
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  • Suresh K. Mohankumar
    Affiliations
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada

    Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB, Canada
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  • Jaime L. Clark
    Affiliations
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada

    Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB, Canada
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  • Carla G. Taylor
    Correspondence
    Corresponding author at: St. Boniface Hospital Research Centre, R2034-351 Tache Avenue, Winnipeg, MB, Canada R2H 2A6. Tel.: +1 204 258 1361; fax: +1 204 237 4018.
    Affiliations
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada

    Department of Physiology, University of Manitoba, Winnipeg, MB, Canada

    Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB, Canada
    Search for articles by this author
Published:October 21, 2013DOI:https://doi.org/10.1016/j.plefa.2013.09.009

      Abstract

      This study investigated the efficacy of the plant-based n−3 fatty acid, α-linolenic acid (ALA), a dietary precursor of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for modulating hepatic steatosis. Rats were fed high fat (55% energy) diets containing high oleic canola oil, canola oil, a canola/flax oil blend (C/F, 3:1), safflower oil, soybean oil, or lard. After 12 weeks, C/F and weight-matched (WM) groups had 20% less liver lipid. Body mass, liver weight, glucose and lipid metabolism, inflammation and molecular markers of fatty acid oxidation, synthesis, desaturation and elongation did not account for this effect. The C/F group had the highest total n−3 and EPA in hepatic phospholipids (PL), as well as one of the highest DHA and lowest arachidonic acid (n−6) concentrations. In conclusion, the C/F diet with the highest content of the plant-based n−3 ALA attenuated hepatic steatosis and altered the hepatic PL fatty acid profile.

      Abbreviations:

      (ACC1) (acetyl-CoA carboxylase 1), (ACOX1) (acetyl-CoA oxidase 1), (ALA) (α-linolenic acid), (ALT) (alanine aminotransferase), (AST) (aspartate aminotransferase), (DIO) (diet-induced obese), (EPA) (eicosapentaenoic acid), (DHA) (docosahexaenoic acid), (HOMA-IR) (homeostasis model assessment-insulin resistance), (MCP-1) (monocyte chemoattractant protein-1), (MUFA) (monounsaturated fatty acids), (NAFLD) (non-alcoholic fatty liver disease), (PPAR-α) (peroxisome proliferator activated receptor α), (PL) (phospholipids), (PUFA) (polyunsaturated fatty acids), (SFA) (saturated fatty acids), (SREBP-1c) (sterol regulatory element-binding protein-1c), (TAG) (triacylglycerols)

      Keywords

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      References

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