Abstract
Our recent study indicates that maternal vitamin B12 and omega-3 fatty acid status influence plasma and erythrocyte fatty acid profile
in dams. The present study examines the effects of prenatal and postnatal vitamin
B12 and omega-3 fatty acid status on lipid metabolism in the offspring. Pregnant dams
were divided into five groups: Control; Vitamin B12 deficient (BD); Vitamin B12 supplemented (BS); Vitamin B12 deficient group supplemented with omega-3 fatty acids (BDO); Vitamin B12 supplemented group with omega-3 fatty acids (BSO). The offspring were continued on
the same diets till 3 month of age. Vitamin B12 deficiency increased cholesterol levels (p<0.01) but reduced docosahexaenoic acid (DHA) (p<0.05), liver mRNA levels of acetyl CoA carboxylase-1 (ACC-1) (p<0.05) and carnitine palmitoyltransferase-1 (CPT-1) (p<0.01) in the offspring. Omega-3 fatty acid supplementation to this group normalized
cholesterol but not mRNA levels of ACC-1 and CPT-1. Vitamin B12 supplementation normalized the levels cholesterol to that of control but increased
plasma triglyceride (p<0.01) and reduced liver mRNA levels of adiponectin, ACC-1, and CPT-1 (p<0.01 for all). Supplementation of both vitamin B12 and omega-3 fatty acid normalized triglyceride and mRNA levels of all the above genes.
Prenatal and postnatal vitamin B12 and omega-3 fatty acids together play a crucial role in regulating the genes involved
in lipid metabolism in adult offspring.
Abbreviations:
ADP (adiponectin), ACC (acetyl CoAcarboxylase), CPT (carnitine palmitoyltransferase), DHA (docosahexanoic acid), EPA (eicosapentaenoic acid)Keywords
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Article info
Publication history
Published online: April 18, 2015
Accepted:
April 8,
2015
Received in revised form:
April 7,
2015
Received:
November 18,
2014
Identification
Copyright
© 2015 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
