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Abstract
In this study, the changes of arachidonic acid metabolites after an ischemia-reperfusion
(I/R) period are investigated. The cyclooxygenase and lipoxygenase metabolites were
found to be significantly increased after a 45 min period of ischemia followed by
5 min of reperfusion. Prostaglandin E2 (PGE2)- and leukotriene C4 (LTC4)-like activities did not change in the ischemic period, but they both increased after
reperfusion. A cyclooxygenase inhibitor indomethacin and lipoxygenase inhibitor nordehydroguaretic
acid (NDGA) decreased PGE2- and LTC4-like activities, respectively, while allopurinol and superoxide dismutase (SOD) decreased
both activities.
According to our results, it can be assumed that free oxygen radicals are responsible
for the elevation of PGE2- and LTC4-like activities and both of these arachidonic acid metabolites and free oxygen radicals
are the main necrotizing agents in ischemia-reperfusion induced damage.
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Article info
Publication history
Accepted:
February 5,
1996
Received:
September 14,
1995
Identification
Copyright
© 1996 Published by Elsevier Inc.
