Research Article| Volume 59, ISSUE 3, P221-227, September 1998

Ectopic calcification of rat aortas and kidneys is reduced with n-3 fatty acid supplementation

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      Supplementation with essential fatty acids has been shown to prevent the experimentally induced ectopic calcification of the kidneys known as nephrocalcinosis. Male Sprague-Dawley rats were fed a semi-synthetic diet supplemented with different essential fatty acids while being injected for a period of 10 days with calcium glubionate. After 3 weeks their kidneys and aorta were removed and the respective calcium content measured compared to the control, saline injected animals. Lipoic acid-EPA, fish oil (EPA rich) as well as the EPA monoester reduced the calcium concentration of both the kidneys and the aorta towards control values. Lipoic-EPA was the best absorbed of the three compounds and its combination of anti-oxidant together with EPA lowered the calcium content of both the aortas and the kidneys.
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        • Buck A.C
        • Smellie W.S
        • Jenkins R
        • et al.
        The treatment of idiopathic recurrent urolithiasis with fish oil and evening primrose oil — a double-blind study.
        Urolithiasis. 1994; 2: 575-580
        • Kruger M.C
        • Horrobin D.F
        Calcium metabolism, osteoporosis and essential fatty acids: a review.
        Prog Lipid Res. 1997; 36: 131-151
        • Laposata M
        Fatty acids: Biochemistry to clinical significance.
        Am J Clin Pathol. 1995; 104: 172-179
        • Reed L.J
        • de Busk B.G
        • Gunsalus I.C
        • Hormberger Jr, C.S
        Crystalline-lipoic acid: a catalytic agent associated with pyruvate dehydrogenase.
        Science. 1951; 114: 93-94
        • Packer L
        • Wirr E.H
        • Tritscher H.J
        Alpha-lipoic acid as a biological antioxidant (Review article).
        Free Radical Biology & Medicine. 1995; 19: 227-250
        • Haugaard N
        • Haugaard E.S
        Stimulation of glucose utilization by thioctic acid in rat diaphragm incubated in vitro.
        Biochim Biophys Acta. 1970; 222: 583-586
        • Burgess N.A
        • Reynolds T.M
        • Williams N
        • et al.
        Evaluation of four animal models of intrarenal calcium deposition and assessment of the influence of dietary supplementation with essential fatty acids on calcification.
        Urol Res. 1995; 23: 239-242
        • Buck A.C
        • Davies R.L.I
        • Leaker B
        • Moffat D.B
        Inhibition of experimental nephrocalcinosis with prostaglandin synthetase inhibitor.
        J Urol. 1991; 146: 188-194
        • Buck A.C
        • Sampson W.F
        • Lote C.J
        • Blacklock N.J
        The influence of renal prostaglandins on glomerular filtration rate (GFR) and calcium excretion in urolithiasis.
        Br J Urol. 1981; 53: 485-491
        • Tulloch I
        • Smellie W.S.A
        • Buck A.C
        Evening primrose oil reduces urinary calcium excretion in both normal and hypercalciuric rats.
        Urol Res. 1994; 22: 227-230
        • Rose H.G
        • Oklander M
        Improved method for the extraction of lipids from human erythrocytes.
        J Lipid Res. 1965; 6: 428-431
        • Steck T.L
        • Kant J.A
        Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes.
        Methods Enzymol. 1974; 31 (Part A): 172-180
        • Burton G.W
        • Ingold K.U
        • Thompson K.E
        An improved procedure for the isolation of ghost membranes from human red blood cells [letter].
        Lipids. 1981; 16: 946
        • Folch J
        • Lees M
        • Stanley G.H
        A simple method for the isolation and purification of total lipids from animal tissues.
        J Biol Chem. 1957; 226: 497-509
        • Smuts C.M
        • Kruger M
        • Van Jaarsveld P.J
        • et al.
        The influence of fish oil supplementation on plasma lipoproteins and arterial lipids in velvet monkeys with established atherosclerosis.
        Prostaglandins Leukot Essent Fatty Acids. 1992; 47: 129-138